Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000871" target="_blank" >RIV/00027162:_____/12:#0000871 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jviromet.2011.12.007" target="_blank" >http://dx.doi.org/10.1016/j.jviromet.2011.12.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jviromet.2011.12.007" target="_blank" >10.1016/j.jviromet.2011.12.007</a>

Result language
angličtina
Original language name
Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples
Original language description
The aim of this study was to optimise a two-tube reverse transcription triplex quantitative real time PCR (qRT-PCR) combining amplification of two loci with an internal amplification control (IAC) for detection and quantitation of hepatitis E virus (HEV)RNA and to validate its performance on a pool of biological samples. Optimisation was performed on serially diluted "home-made" RNA standards. The limit of detection was determined experimentally as 10 copies/mu l of the RNA standard for both amplification targets. The qRT-PCR was validated on a cohort of samples originating from 48 wild boars (Sus scrofa), 17 fallow deer (Dama dama) and 28 mouflons (Ovis musimon), with nested RT-PCR used as a reference method. qRT-PCR was found to be more specific forthe detection of HEV RNA in examined samples. HEV RNA was detected in samples of five more animals (one wild boar and four mouflons) in comparison with nested RT-PCR. (C) 2011 Elsevier B.V. All rights reserved.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)