CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000917" target="_blank" >RIV/00027162:_____/12:#0000917 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.cyto.2012.01.004" target="_blank" >http://dx.doi.org/10.1016/j.cyto.2012.01.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cyto.2012.01.004" target="_blank" >10.1016/j.cyto.2012.01.004</a>

Result language

angličtina

Original language name

CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine

Original language description

In the veterinary field, only limited information is available about interleukin-17A (IL-17), despite the fact that this cytokine plays an important role during pro-inflammatory immune responses and induces the production of chemotactic factors for neutrophils. The aim of this study was to characterize porcine IL-17-producing cells. We tested the cross-reactivity of five anti-human IL-17 monoclonal antibodies because such antibodies against porcine IL-17 are currently unavailable. Whole blood cells (WBCs) were stimulated with phorbol-myristate-acetate (PMA) and ionomycin and subsequently analyzed by flow cytometry. The antibody clone SCPL1362 was found to cross-react with porcine IL-17, whereas the other four antibodies tested did not recognize this cytokine. Using this antibody, we characterized porcine WBC-secreting IL-17 after PMA and ionomycin stimulation. All IL-17-producing WBCs were positive for the T lymphocyte marker CD3. Myeloid cells (CD172 alpha(+)) and B lymphocytes (CD79

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cytokine

ISSN

1043-4666

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

152-157

UT code for WoS article

000303099200003

EID of the result in the Scopus database

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