Male-specific repeats in wild Bovidae

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000950" target="_blank" >RIV/00027162:_____/12:#0000950 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s13353-012-0108-y" target="_blank" >http://dx.doi.org/10.1007/s13353-012-0108-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13353-012-0108-y" target="_blank" >10.1007/s13353-012-0108-y</a>

Result language

angličtina

Original language name

Male-specific repeats in wild Bovidae

Original language description

In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Genetics

ISSN

1234-1983

e-ISSN

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Volume of the periodical

53

Issue of the periodical within the volume

4

Country of publishing house

GE - GEORGIA

Number of pages

11

Pages from-to

423-433

UT code for WoS article

000310227900005

EID of the result in the Scopus database

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