Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001150" target="_blank" >RIV/00027162:_____/14:#0001150 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/14:#0001330
Result on the web
<a href="http://dx.doi.org/10.1016/j.talanta.2014.02.007" target="_blank" >http://dx.doi.org/10.1016/j.talanta.2014.02.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2014.02.007" target="_blank" >10.1016/j.talanta.2014.02.007</a>

Result language
angličtina
Original language name
Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods
Original language description
The analytical performance and evaluation of a kit-based ELISA for the determination of acrylamide in fried potato and corn chip samples are described. The sample homogenate is subjected to clean-up using SPE, followed by analyte derivatization and ELISAdetection. Accuracy, precision and linearity of the ELISA procedure have been validated using spiked samples. Analytical recovery ranged from 91.8% to 96.0% with coefficients of variation below 15%. Good linearity over a wide range of dilution and minimal assay drift was observed within a microtiter plate. IC50 value of the calibration curve was 110 ng/mL, with the limit of detection about 5 ng/mL and dynamic range from 10 to 1000 ng/mL. The high specificity of the ELISA was demonstrated by cross-reactivity study using 11 potential cross-reactants. A good correlation between the results obtained from the ELISA and GC-MS within the concentration range 120-1500 mu g/kg was found in the chip samples (r=0.992, n=120). The data demonstrate
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
<a href="/en/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Center for Advanced Microbiology & Immunology Research in Veterinary Medicine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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