Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001303" target="_blank" >RIV/00027162:_____/15:#0001303 - isvavai.cz</a>
Result on the web
<a href="http://www.futuremedicine.com/loi/fmb" target="_blank" >http://www.futuremedicine.com/loi/fmb</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2217/FMB.14.139" target="_blank" >10.2217/FMB.14.139</a>

Result language
angličtina
Original language name
Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis
Original language description
Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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