Detection of genes associated with developmental competence of bovine oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000038" target="_blank" >RIV/00027162:_____/16:N0000038 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/16:00460683

Result on the web

<a href="https://www.researchgate.net/publication/289584715_Detection_of_genes_associated_with_developmental_competence_of_bovine_oocytes" target="_blank" >https://www.researchgate.net/publication/289584715_Detection_of_genes_associated_with_developmental_competence_of_bovine_oocytes</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2016.01.004" target="_blank" >10.1016/j.anireprosci.2016.01.004</a>

Result language

angličtina

Original language name

Detection of genes associated with developmental competence of bovine oocytes

Original language description

The developmental competence of oocytes is acquired progressively during folliculogenesis and is linked to follicular size. It has been documented that oocytes originating from larger follicles exhibit a greater ability to develop to the blastocyst stage. The differences in cytoplasmic factors such as mRNA transcripts could explain the differences in oocyte developmental potential. We used bovine oligonucleotide microarrays to characterize differences between the gene expression profiles of germinal vesicle stage (GV) oocytes with greater developmental competence from medium follicles (MF) and those with less developmental competence from small follicles (SF). After normalizing the microarray data, our analysis found differences in the level of 60 transcripts (>= 1.4 fold), corresponding to 49 upregulated and 11 downregulated transcripts in MF oocytes compared to SF oocytes. The gene expression data were classified according to gene ontology, the majority of the genes were associated with the regulation of transcription, translation, the cell cycle, and mitochondria) activity. A subset of 16 selected genes was validated for GV oocytes by quantitative real-time RT-PCR; significant differences (P<0.01) were found in the level of TAF1A,MTRF1L, ATP5C1, UBL5 and MAP3K13 between the MF and SF oocytes. After maturation the transcript level remained stable for ATP5F1, BRD7, and UBL5 in both oocyte categories. The transcript level of another 13 genes substantially dropped in the MF and/or SF oocytes. It can be concluded that the developmental competence of bovine oocytes and embryos may be a quantitative trait dependent on small changes in the transcription profiles of many genes).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GI - Farm animal breeding and farm animal pedigree breeding

OECD FORD branch

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Project

<a href="/en/project/LD14104" target="_blank" >LD14104: The effect of mitochondrial activity stimulation on developmental competence of mammalian oocytes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

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Volume of the periodical

Neuveden

Issue of the periodical within the volume

166

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

58-71

UT code for WoS article

000371189400008

EID of the result in the Scopus database

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