2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000040" target="_blank" >RIV/00027162:_____/16:N0000040 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/16:00463844 RIV/61989592:15310/16:33161238

Result on the web

<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0159269" target="_blank" >http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0159269</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0159269" target="_blank" >10.1371/journal.pone.0159269</a>

Result language

angličtina

Original language name

2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis

Original language description

The herbicide 2,4-D exhibits an auxinic activity and therefore can be used as a synthetic and traceable analog to study auxin-related responses. Here we identified that not only exogenous 2,4-D but also its amide-linked metabolite 2,4-D-Glu displayed an inhibitory effect on plant growth via the TIR1/AFB auxin-mediated signaling pathway. To further investigate 2,4-D metabolite conversion, identity and activity, we have developed a novel purification procedure based on the combination of ion exchange and immuno-specific sorbents combined with a sensitive liquid chromatography-mass spectrometry method. In 2,4-D treated samples, 2,4-D-Glu and 2,4-D-Asp were detected at 100-fold lower concentrations compared to 2,4-D levels, showing that 2,4-D can be metabolized in the plant. Moreover, 2,4-D-Asp and 2,4-D-Glu were identified as reversible forms of 2,4-D homeostasis that can be converted to free 2,4-D. This work paves the way to new studies of auxin action in plant development.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plos One

ISSN

1932-6203

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

18

Pages from-to

nestrankovano

UT code for WoS article

000380169600044

EID of the result in the Scopus database

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