3D rotating wall vessel and 2D cell culture of four veterinary virus pathogens: a comparison of virus yields, portions of infectious particles and virus growth curves

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000067" target="_blank" >RIV/00027162:_____/16:N0000067 - isvavai.cz</a>

Result on the web

<a href="http://www.journals.elsevier.com/journal-of-virological-methods/" target="_blank" >http://www.journals.elsevier.com/journal-of-virological-methods/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jviromet.2015.11.002" target="_blank" >10.1016/j.jviromet.2015.11.002</a>

Result language

angličtina

Original language name

3D rotating wall vessel and 2D cell culture of four veterinary virus pathogens: a comparison of virus yields, portions of infectious particles and virus growth curves

Original language description

Only very few comparative studies have been performed that evaluate general trends of virus growth under 3D in comparison with 2D cell culture conditions. The aim of this study was to investigate differences when four animal viruses are cultured in 2D and 3D. Suid herpesvirus 1 (SuHV-1), Vesicular stomatitis virus (VSIV), Bovine adenovirus (BAdV) and Bovine parainfluenza 3 virus (BPIV-3) were cultivated in 3D rotating wall vessels (RWVs) and conventional 2D cultures. The production of virus particles, the portion of infectious particles, and the infectious growth curves were compared. For all viruses, the production of virus particles (related to cell density), including the non-infectious ones, was lower in 3D than in 2D culture. The production of only infectious particles was significantly lower in BAdV and BPIV-3 in 3D cultures in relation to cell density. The two cultivation approaches resulted in significantly different virus particle-to-TCID50 ratios in three of the four viruses: lower in SuHV-1 and BPIV-3 and higher in BAdV in 3D culture. The infectious virus growth rates were not significantly different in all viruses. Although 3D RWV culture resulted in lower production of virus particles compared to 2D systems, the portion of infectious particles was higher for some viruses.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/LO1218" target="_blank" >LO1218: Healthy animal as a source of wholesome food</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Virological Methods

ISSN

0166-0934

e-ISSN

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Volume of the periodical

228

Issue of the periodical within the volume

FEB

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

10-15

UT code for WoS article

000369679200002

EID of the result in the Scopus database

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