Prevalence and characteristics of Salmonella species isolated from captive reptiles in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000025" target="_blank" >RIV/00027162:_____/17:N0000025 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/17:43875880

Result on the web

<a href="http://www.agriculturejournals.cz/publicFiles/223420.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/223420.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/44/2017-VETMED" target="_blank" >10.17221/44/2017-VETMED</a>

Result language

angličtina

Original language name

Prevalence and characteristics of Salmonella species isolated from captive reptiles in the Czech Republic

Original language description

This study was aimed at determining the prevalence and characterising the strains of Salmonella species in various captive reptiles in the Czech Republic. A total of 211 samples of cloacal swabs from lizards, chelonians and snakes, and 14 swabs from terraria surfaces were collected between November 2014 and July 2015. After isolation according to the reference method (EN ISO), Salmonella spp. isolates were characterised using serotyping and macrorestriction analysis followed by pulsed field gel electrophoresis and antimicrobial susceptibility testing. Altogether, 39 isolates were obtained from 29 (19%) reptiles and from terraria surfaces. Among the different reptilian species, Salmonella spp. were found in 22 (25.6%) lizards, three (17.6%) snakes and four (8%) chelonians with 31 isolates classified as Salmonella enterica subspecies enterica and eight isolates classified as Salmonella enterica subsp. salamae. In total, 14 different serotypes were detected, with the most frequent serotypes being Salmonella Oranienburg, S. Fluntern, S. Tennessee and S. Cotham. Resistance to one antimicrobial agent (ampicillin, tetracycline or streptomycin) was detected in five isolates. The results of the macrorestriction analysis within the serotype groups showed varying level of heterogeneity. This study confirms that reptiles kept as pets can be both carriers and reservoirs of Salmonella spp., and that they can harbour various serotypes with intermittent excretion of the bacteria in faeces. Half of the detected serotypes have been involved in human reptileassociated salmonellosis cases in the past. S. enterica subsp. salamae serotype O:1,13,23;H: z29;H:1,5, monophasic S. enterica subsp. salamae serotype O:40;H:g,t; H:-and its biphasic form (S. enterica subsp. salamae serotype O:40; H:g,t;H:1,5) have apparently been isolated from reptiles for the first time in this study.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/LO1218" target="_blank" >LO1218: Healthy animal as a source of wholesome food</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

—

Volume of the periodical

62

Issue of the periodical within the volume

8

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

14

Pages from-to

456-469

UT code for WoS article

000408605000005

EID of the result in the Scopus database

—