Molecular Characterization of OXA-48-Like- Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000049" target="_blank" >RIV/00027162:_____/17:N0000049 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11140/17:10331747 RIV/00216208:11150/17:10331747 RIV/75010330:_____/17:00011790

Result on the web

<a href="http://aac.asm.org/content/early/2016/11/08/AAC.01889-16.abstract" target="_blank" >http://aac.asm.org/content/early/2016/11/08/AAC.01889-16.abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AAC.01889-16" target="_blank" >10.1128/AAC.01889-16</a>

Result language

angličtina

Original language name

Molecular Characterization of OXA-48-Like- Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids

Original language description

The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in the Czech Republic. From 2013 to 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli isolates, and 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized or infected by two or three different OXA-48-like producers. The K. pneumoniae isolates were classified into nine sequence types (STs), with ST101 being predominant (n = 8). The E. coli isolates were of different STs, while the E. cloacae isolate belonged to ST109. Twenty-four isolates carried bla(OXA-48), while two isolates carried bla(OXA-181) or bla(OXA-232). Almost all isolates (n = 22) carried bla(OXA-48)-positive plasmids of a similar size (similar to 60 kb), except the two isolates producing OXA-181 or OXA-232. In an ST45 K. pneumoniae isolate and an ST38 E. coli isolate, S1 nuclease profiling plus hybridization indicated a chromosomal location of bla(OXA-48). Sequencing showed that the majority of bla(OXA-48)-carrying plasmids exhibited high degrees of identity with the pOXA-48-like plasmid pE71T. Additionally, two novel pE71T derivatives, pOXA48_ 30715 and pOXA-48_30891, were observed. The bla(OXA-181)-carrying plasmid was identical to the IncX3 plasmid pOXA181_EC14828, while the bla(OXA-232)-carrying plasmid was a ColE2-type plasmid, being a novel derivative of pOXA-232. Finally, sequencing data showed that the ST45 K. pneumoniae and ST38 E. coli isolates harbored the IS1R-based composite transposon Tn6237 containing bla(OXA-48) integrated into their chromosomes. These findings underlined that the horizontal transfer of pOXA-48-like plasmids has played a major role in the dissemination of bla(OXA-48) in the Czech Republic. In combination with the difficulties with their detection, OXA-48 producers constitute an important public threat.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30302 - Epidemiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Antimicrobial Agents and Chemotherapy

ISSN

0066-4804

e-ISSN

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Volume of the periodical

61

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

e01889-16

UT code for WoS article

000394102500045

EID of the result in the Scopus database

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