First description in Europe of the emergence of Enterococcus faecium ST117 carrying both vanA and vanB genes, isolated in Greece

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000074" target="_blank" >RIV/00027162:_____/17:N0000074 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11140/17:10365179

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S2213716517301388?via%3Dihub" target="_blank" >http://www.sciencedirect.com/science/article/pii/S2213716517301388?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jgar.2017.07.010" target="_blank" >10.1016/j.jgar.2017.07.010</a>

Result language

angličtina

Original language name

First description in Europe of the emergence of Enterococcus faecium ST117 carrying both vanA and vanB genes, isolated in Greece

Original language description

OBJECTIVES: An Enterococcus faecium isolate (Efa-125) carrying both the vanA and vanB genes was recovered from a patient with bacteraemia treated in a Greek hospital. Since this is the first description in Europe of E. faecium carrying both vanA and vanB genes, the isolate was further studied. METHODS: Susceptibility to several antibiotics was determined using the VITEK®2 automated system. The isolate was typed by multilocus sequence typing (MLST). To define the genetic units of the vanA and vanB genes, the plasmid content of Efa-125 was analysed by pulsed-field gel electrophoresis (PFGE) of total DNA digested with S1 nuclease followed by hybridisation with digoxigenin-labelled vanA and vanB probes. In addition, plasmids and chromosomes were sequenced using the Illumina MiSeq platform. RESULTS: E. faecium Efa-125 belonged to ST117 and expressed resistance both to vancomycin and teicoplanin, with minimum inhibitory concentrations (MICs) for both of 256mg/L. The vanA gene was carried on a 29 320-bp plasmid exhibiting high similarity to pA6981 previously characterised from Enterococcus gallinarum A6981, whereas vanB was part of a Tn1549-like transposon integrated into the chromosome. Expression of the VanA phenotype was correlated with the presence of intact vanZ and vanS genes. CONCLUSIONS: This is the first detection in Greece of vanA-vanB genotype/VanA phenotype E. faecium and indicates an evolving epidemiology of vancomycin-resistant enterococci.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30302 - Epidemiology

Project

<a href="/en/project/NV15-28663A" target="_blank" >NV15-28663A: Molecular-Epidemiological Typing of Multiresistant Enterobacteriaceae and Pseudomonas spp. with Special Focus on their Silent Sources</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Global Antimicrobial Resistance

ISSN

2213-7165

e-ISSN

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Volume of the periodical

—

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

3

Pages from-to

68-70

UT code for WoS article

000416992000014

EID of the result in the Scopus database

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