Characterisation of Zika virus infection in primary human astrocytes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000072" target="_blank" >RIV/00027162:_____/18:N0000072 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00498814 RIV/60076658:12310/18:43897581
Result on the web
<a href="https://bmcneurosci.biomedcentral.com/articles/10.1186/s12868-018-0407-2" target="_blank" >https://bmcneurosci.biomedcentral.com/articles/10.1186/s12868-018-0407-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12868-018-0407-2" target="_blank" >10.1186/s12868-018-0407-2</a>

Result language
angličtina
Original language name
Characterisation of Zika virus infection in primary human astrocytes
Original language description
BACKGROUND: The recent Zika virus (ZIKV) outbreak has linked ZIKV with microcephaly and other central nervous system pathologies in humans. Astrocytes are among the first cells to respond to ZIKV infection in the brain and are also targets for virus infection. In this study, we investigated the interaction between ZIKV and primary human brain cortical astrocytes (HBCA). RESULTS: HBCAs were highly sensitive to representatives of both Asian and African ZIKV lineages and produced high viral yields. The infection was associated with limited immune cytokine/chemokine response activation; the highest increase of expression, following infection, was seen in CXCL-10 (IP-10), interleukin-6, 8, 12, and CCL5 (RANTES). Ultrastructural changes in the ZIKV-infected HBCA were characterized by electron tomography (ET). ET reconstructions elucidated high-resolution 3D images of the proliferating and extensively rearranged endoplasmic reticulum (ER) containing viral particles and virus-induced vesicles, tightly juxtaposed to collapsed ER cisternae. CONCLUSIONS: The results confirm that human astrocytes are sensitive to ZIKV infection and could be a source of proinflammatory cytokines in the ZIKV-infected brain tissue.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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