Characterization of blaKPC-3-positive plasmids from an Enterobacter aerogenes isolated from a corvid in Canada
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000121" target="_blank" >RIV/00027162:_____/18:N0000121 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/18:10376553 RIV/62157124:16270/18:43876612 RIV/62157124:16810/18:43876612
Result on the web
<a href="https://academic.oup.com/jac/article-abstract/73/9/2573/5026317?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/jac/article-abstract/73/9/2573/5026317?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jac/dky199" target="_blank" >10.1093/jac/dky199</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of blaKPC-3-positive plasmids from an Enterobacter aerogenes isolated from a corvid in Canada
Original language description
To examine the environmental contamination by carbapenemase-producing Enterobacteriaceae (CPE) in Canada, total of 449 faecal samples from wild corvids were analyzed. Carbapenemase production was hypothesized by MALDI-TOF MS meropenem hydrolysis assay. Carbapenemase-encoding genes were detected by PCR. Transfer experiments by conjugation were carried out with Escherichia coli recipients. blaKPC-carrying plasmids were characterized by S1 profiling and Illumina sequencing. Among 242 crbapenem-non-susceptible Gram-negative bacteria, only one KPC-3-producing Enterobacter aerogenes isolate (Eaer-4382) was identified. blaKPC-3 was transferred to E.coli laboratory strain by conjugation, while S1 analysis revealed the presence of two types of transconjugants carrying blaKPC-positive plasmids of different sizes (~90-kb [pEaer-4382s] or ~120-kb [pEaer-4382b]). pEaer-4382b (134521 bp) consisted of an IncFII-type sequence (94320 bp) and an acquired sequence (40201 bp) including a truncated KPC-3-encoding Tn4401b transposon. Plasmid pEaer-4382s (108772 bp) differed by a 25638-bp deletion of the acquired region of pEaer-4382b. Additionally, an 8-bp duplication (ACAAGATA) was identified in the region, between the resD and parA genes, resulting in a different number of iterons. Even only one KPC-3 producer was isolated from a wild corvid, the findings of the present study confirmed the environmental contamination by CPE in Canada. Furthermore, sequencing data underlined the ongoing evolution of blaKPC-carrying mobile elements.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Antimicrobial Chemotherapy
ISSN
0305-7453
e-ISSN
1460-2091
Volume of the periodical
73
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
3
Pages from-to
2573–2575
UT code for WoS article
000443539100043
EID of the result in the Scopus database
—