Mannan-coated nanoliposome prepared via orthogonal aminooxy lipid-based click chemistry and microfluidics mixing: characterization of the structure and in vitro biological activities

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000144" target="_blank" >RIV/00027162:_____/18:N0000144 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Mannan-coated nanoliposome prepared via orthogonal aminooxy lipid-based click chemistry and microfluidics mixing: characterization of the structure and in vitro biological activities

Original language description

Poster. New synthetic aminooxy lipid was used as building block for the formulation of functionalised nanoliposomes by microfluidic mixing. Outer surface of functionalised nanoliposomes was modified by orthogonal binding of reducing termini of cellular mannan (Candida glabrata (CCY 26-20-1) to oxime lipids via click chemistry reaction based on aminooxy coupling (oxime ligation). Aminooxy lipid was proved as suitable active component for preparation of functionalised nanoliposomes by the microfluidic mixing method. The structure of resulting mannan-liposomes were visualized by transmission (TEM) and scanning (SEM) electron microscopy as well as by dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), and tunable resistive pulse sensing (TPRS). In vitro experiments on human and mouse dendritic cells demonstrate selective internalization of fluorochrome-labelled mannan-liposomes and their ability to stimulate DC comparable to lipopolysaccharide. We described a potentially new drug delivery platform for mannan receptor-targeted antimicrobial drugs as well as for immunotherapeutics. Furthermore, the platform based on mannans bound orthogonally onto the surface of nanoliposomes represents self-adjuvanted carrier for construction of liposome-based recombinant vaccines for both systemic and mucosal route of administration.

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/EF15_003%2F0000495" target="_blank" >EF15_003/0000495: FIT (Pharmacology, Immunotherapy, nanoToxicology)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů