Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000172" target="_blank" >RIV/00027162:_____/18:N0000172 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/18:10381049 RIV/00216208:11150/18:10381049 RIV/00179906:_____/18:10381049 RIV/75010330:_____/18:00012186
Result on the web
<a href="http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000765" target="_blank" >http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000765</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/jmm.0.000765" target="_blank" >10.1099/jmm.0.000765</a>
Alternative languages
Result language
angličtina
Original language name
Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era
Original language description
The aim of this study was to characterize serogroup 19 isolates resistant to macrolides and/or penicillin found among pneumococci recovered from cases of invasive and respiratory tract disease in the Czech Republic in 2014.Pneumococcal isolates of serotypes 19A (n=26) and 19F (n=10) that were non-susceptible to penicillin and/or macrolides and had been collected in 2014 were analysed using multi-locus sequence typing (MLST). Four isolates representing the major clones were subjected to whole-genome sequencing (WGS). The penicillin-susceptible macrolide-resistant isolates of serotype 19A were mainly associated with sequence type (ST) 416 belonging to clonal complex (CC) 199, and the penicillin-resistant isolates were of serotype 19F belonging to ST1464 (CC 320). WGS revealed the presence of pilus 1, in association with pilus 2, in serotype19F isolates belonging to CC 320. Another adhesin, pneumococcal serine-rich protein (PsrP), was only present in serotype 19A isolates of ST416. Analysis of the penicillin-binding proteins (PBPs) of serotype 19F penicillin-resistant isolates (ST1464 and ST271) performed on PBP1a, 2b and 2x identified a large number of mutations in comparison to the reference strain, R6. Both isolates contained a unique PBP profile; however, they were highly similar to PBP sequences of the Taiwan19F-14 reference strain. The Pbp2b sequences of both 19F isolates showed the lowest similarity to those of the Taiwan19F-14 strain (91 % similarity), while they were also found to be distantly related to each other (94 % similarity). WGS revealed specific virulence factors in antibiotic-resistant pneumococcal clones that spread rapidly in the post-vaccine era in the Czech Republic.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/NV16-27109A" target="_blank" >NV16-27109A: Evolution of emergent multidrug resistant Streptococcus pneumoniae 19A serotype in era of pneumococcal vaccination.</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Medical Microbiology
ISSN
0022-2615
e-ISSN
1473-5644
Volume of the periodical
67
Issue of the periodical within the volume
7
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
1003-1011
UT code for WoS article
000437235000016
EID of the result in the Scopus database
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