Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000226" target="_blank" >RIV/00027162:_____/18:N0000226 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/18:00491108 RIV/00216224:14310/18:00102187
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26641" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26641</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcb.26641" target="_blank" >10.1002/jcb.26641</a>
Alternative languages
Result language
angličtina
Original language name
Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells
Original language description
Docosahexaenoic acid (DHA) and sodium butyrate (NaBt) exhibit a number of interactive effects on colon cancer cell growth, differentiation or apoptosis; however, the molecular mechanisms responsible for these interactions and their impact on cellular lipidome are still not fully clear. Here, we show that both dietary agents together induce dynamic alterations of lipid metabolism, specific cellular lipid classes and fatty acid composition. In HT-29 cell line, a model of differentiating colon carcinoma cells, NaBt supported incorporation of free DHA into non-polar lipids and their accumulation in cytoplasmic lipid droplets. DHA itself was not incorporated into sphingolipids; however, it significantly altered representation of individual ceramide (Cer) classes, in particular in combination with NaBt (DHA/NaBt). We observed altered expression of enzymes involved in Cer metabolism in cells treated with NaBt or DHA/NaBt, and exogenous Cer 16:0 was found to promote induction of apoptosis in differentiating HT-29 cells. NaBt, together with DHA, increased n-3 fatty acid synthesis and attenuated metabolism of monounsaturated fatty acids. Finally, DHA and/or NaBt altered expression of proteins involved in synthesis of fatty acids, including elongase 5, stearoyl CoA desaturase 1 or fatty acid synthase, with NaBt increasing expression of caveolin-1 and CD36 transporter, which may further promote DHA incorporation and its impact on cellular lipidome. In conclusion, our results indicate that interactions of DHA and NaBt exert complex changes in cellular lipidome, which may contribute to the alterations of colon cancer cell differentiation/apoptotic responses, which extends our knowledge about the nature of interactive effects of dietary fatty acids. This article is protected by copyright. All rights reserved
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cellular Biochemistry
ISSN
0730-2312
e-ISSN
1097-4644
Volume of the periodical
119
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
4664-4679
UT code for WoS article
000430667200041
EID of the result in the Scopus database
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