Multiple virus infections in Western honeybee (Apis mellifera L.) ejaculate used for instrumental insemination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000006" target="_blank" >RIV/00027162:_____/19:N0000006 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/20:80497

Result on the web

<a href="https://www.mdpi.com/1999-4915/11/4/306" target="_blank" >https://www.mdpi.com/1999-4915/11/4/306</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v11040306" target="_blank" >10.3390/v11040306</a>

Result language

angličtina

Original language name

Multiple virus infections in Western honeybee (Apis mellifera L.) ejaculate used for instrumental insemination

Original language description

Instrumental insemination of Apis mellifera L. queens is widely employed technique used in honeybee breeding which enables effective control of mating. However, drone semen represents potential source of honeybee viruses. In this study, 43 semen doses collected from apparently healthy drones, and consequently used in instrumental insemination, were analysed using PCR or RT-PCR to detect the presence of viral genome of eleven honeybee viruses. In 90.7% of samples, viral infection was detected. The survey revealed genomes of five viruses, namely Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Sacbrood virus (SBV), and A. mellifera filamentous virus (AmFV) in 83.7%, 18.6%, 13.9%, 2.3%, and 67.4% of samples, respectively. Single infection (30.2% of samples) as well as multiple infection (60.5% of samples) of two, three or four pathogens were also evaluated. To the best of our knowledge, this is the first study describing the presence of the BQCV and SBV genome sequence in drone ejaculate. Phylogenetic analysis of BQCV partial helicase gene sequence revealed high similarity of nucleotide sequence of described Czech strains, which varied from 91.4% to 99.6%. The findings of our study indicate the possibility of venereal transmission of BQCV and SBV.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Viruses

ISSN

1999-4915

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

306

UT code for WoS article

000467310600003

EID of the result in the Scopus database

2-s2.0-85064209954