Evaluation of colostrum quality in the Czech Republic using radial immunodiffusion and different types of refractometers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000008" target="_blank" >RIV/00027162:_____/19:N0000008 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/19:43878131 RIV/00027014:_____/19:N0000017

Result on the web

<a href="https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=122_2018-VETMED" target="_blank" >https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=122_2018-VETMED</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/122/2018-VETMED" target="_blank" >10.17221/122/2018-VETMED</a>

Result language

angličtina

Original language name

Evaluation of colostrum quality in the Czech Republic using radial immunodiffusion and different types of refractometers

Original language description

The objectives of this study were to determine the immunoglobulin G concentration of colostrum in Czech dairy cows, to compare refractometer results with results achieved using the radial immunodiffusion method and to evaluate the reliability of three types of refractometers and recommend the best solution for the evaluation of colostrum quality. Colostrum samples (n = 1522) were collected from 38 herds between 2015 and 2017. The immunological quality of colostrum was estimated using Brix refractometers (optical, simple digital, digital Misco) and compared with the immunoglobulin G concentration assessed using radial immunodiffusion. We found high variability in the quality of colostrum. The minimum, maximum and median of individual measurements were the following: radial immunodiffusion immunoglobulin G – 5.2, 199.1, 76.9 g/l; optical refractometer – 9.5, 32.0, 23.1% Brix; simple digital refractometer – 5.4, 35.0, 19.1% Brix; digital refractometer Misco – 9.8, 37.4, 23.2% Brix. On the basis of immunoglobulin G concentration assessed using radial immunodiffusion, 20.9% of colostrum samples were of low quality (immunoglobulin G < 50 g/l). The Spearman correlation coefficients between radial immunodiffusion and the Brix refractometer readings were 0.62–0.67 (P < 0.001) according to the type of refractometer. The cut-off evaluation of the readings from optical and Misco digital refractometers both showed 20% Brix, with sensitivities of 89.4% and 88.2%, specificities of 73.2% and 74.5% and accuracies of 86.0% and 85.4%, respectively. The cut-off level for the simple digital refractometer showed 17% Brix with a sensitivity of 77.5%, specificity of 80.4% and an insufficient accuracy of 78.1%. For optical and Misco refractometers we recommend the use of two cut-off levels for the evaluation of colostrum: 23% Brix for the selection of good quality colostrum suitable for freezing and 19% Brix to discard poor quality colostrum. The different cut-off levels obtained by measuring with different types of refractometers indicate the need to check the quality of the instruments prior to their use in practice and, where appropriate, to determine their cut-off levels by comparison with results obtained using the reference method.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

—

Volume of the periodical

64

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

51-59

UT code for WoS article

000459023400001

EID of the result in the Scopus database

2-s2.0-85063737759