Sex determining region Y (SRY) sequencing and non-invasive molecular sexing in three wild species: brown (Parahyaena brunnea) and spotted (Crocuta crocuta) hyenas and aardvark (Orycteropus afer)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000209" target="_blank" >RIV/00027162:_____/19:N0000209 - isvavai.cz</a>
Result on the web
<a href="http://www.publish.csiro.au/rd/RD18468" target="_blank" >http://www.publish.csiro.au/rd/RD18468</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1071/RD18468" target="_blank" >10.1071/RD18468</a>
Alternative languages
Result language
angličtina
Original language name
Sex determining region Y (SRY) sequencing and non-invasive molecular sexing in three wild species: brown (Parahyaena brunnea) and spotted (Crocuta crocuta) hyenas and aardvark (Orycteropus afer)
Original language description
Noninvasive molecular sexing methods are useful in captive breeding programmes and in field studies. They enable sex identification without immobilisation or stressful handling of the animals. We developed a method enabling fast and reliable sex identification in three species with limited external sexual dimorphism: the brown and spotted hyenas and the aardvark. We used the SRY gene as the male-specific sequence and the c-myc gene, highly conserved among mammals, as the control sequence present in both sexes. Primers designed on the basis of the feline and human SRY gene enabled us to amplify and sequence the SRY gene fragment in hyenas and in the aardvark, respectively. Subsequently, we used specific primers designed on the basis of the newly obtained sequences for sex determination in two brown hyenas, three spotted hyenas and six aardvarks. The sequences of the SRY gene fragments were further studied. Interspecies comparisons revealed high similarity between the SRY sequences of both hyena species and their relationships with the Felidae lineage. The aardvark, as the only species of the order Tubulidentata (Afrotheria), showed SRY gene similarities with Sirenia and Primates. The knowledge of phylogenetic relationships can be beneficial in genetic studies focused on species with limited sequence data.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reproduction, Fertility and Development
ISSN
1031-3613
e-ISSN
1448-5990
Volume of the periodical
31
Issue of the periodical within the volume
8
Country of publishing house
AU - AUSTRALIA
Number of pages
5
Pages from-to
1419-1423
UT code for WoS article
000475318800013
EID of the result in the Scopus database
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