Paralogs vs. genotypes? Variability of Babesia canis assessed by 18S rDNA and two mitochondrial markers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000222" target="_blank" >RIV/00027162:_____/19:N0000222 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/19:00519677 RIV/62157124:16170/19:43877308 RIV/62157124:16810/19:43877308
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0304401719300172?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0304401719300172?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vetpar.2018.12.017" target="_blank" >10.1016/j.vetpar.2018.12.017</a>
Alternative languages
Result language
angličtina
Original language name
Paralogs vs. genotypes? Variability of Babesia canis assessed by 18S rDNA and two mitochondrial markers
Original language description
Canine babesiosis caused by Babesia canis sensu stricto became an emerging disease of dogs across Europe calling for attention also in countries where it was an only rare imported disease. An easy accessibility of molecular methods and the growing amount of sequencing data led to the description of intraspecific variability in 18S rDNA sequences designated as "genotypes". Using material from a homogenous cohort of dogs with microscopically confirmed canine babesiosis caused by B. canis, we evaluated Babesia intraspecific variability and amplification sensitivity of three different genes (18S rDNA, COI, Cytb) to assess their potential as diagnostic or phylogenetic markers. In raw sequencing data obtained, we observed at least 3 ambiguous positions in up to 86% of chromatograms within the ∼560 bp fragment of 18S rDNA suggesting the existence of several, not identical copies of this gene. Our COI haplotype analysis resulted in a star-like pattern indicating a recent origin of most haplotypes, but not supporting the existence of two dominant haplotypes. Similarly, the Cytb sequences obtained from samples with all variants of 18S rDNA were identical. We corroborate previous observations from three other European countries and bring the evidence of the existence of 18S rDNA paralogs in B. canis genome replacing currently used “genotype” theory.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Veterinary Parasitology
ISSN
0304-4017
e-ISSN
1873-2550
Volume of the periodical
266
Issue of the periodical within the volume
FEB 2019
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
103-110
UT code for WoS article
000459643700015
EID of the result in the Scopus database
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