Systematic Culturomics Shows that Half of Chicken Caecal Microbiota Members can be Grown in Vitro Except for Two Lineages of Clostridiales and a Single Lineage of Bacteroidetes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000335" target="_blank" >RIV/00027162:_____/19:N0000335 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/19:43877837 RIV/62157124:16810/19:43877837

Result on the web

<a href="https://www.ncbi.nlm.nih.gov/pubmed/31661802" target="_blank" >https://www.ncbi.nlm.nih.gov/pubmed/31661802</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms7110496" target="_blank" >10.3390/microorganisms7110496</a>

Result language

angličtina

Original language name

Systematic Culturomics Shows that Half of Chicken Caecal Microbiota Members can be Grown in Vitro Except for Two Lineages of Clostridiales and a Single Lineage of Bacteroidetes

Original language description

Epidemiological data show that composition of gut microbiota influence host health or disease status, age or even behaviour. However, to confirm epidemiological observation in controlled experiments, pure cultures of gut anaerobes must be obtained. Since this is not a simple task due to large number of bacterial species colonising intestinal tract, in this study we inoculated 174 different culture media with caecal samples from adult hens, and compared microbiota composition in the original caecal samples and in bacterial mass growing in vitro by 16S rRNA sequencing. We observed that 42 % of gut microbiota members could be grown in vitro and since there were some species which we did not culture but for which the culture conditions are known, it is likely that more than half of chicken gut microbiota can be grown in vitro. However, there were two lineages of Clostridiales and a single lineage of Bacteroidetes which were common to caecal microbiota but resistant to culture. Of the most selective culture conditions, nutrient broths supplemented with mono- or disaccharides incl. those present in fruits positively selected for Lactobacillaceae. Addition of bile salts selected for Veillonellaceae and use of YFCA enriched for Desulfovibrionaceae. In addition, Erysipelotrichaceae were positively selected by colistin, trimethoprim, streptomycin and nalidixic acid. Such culture conditions can be therefore used for selective enrichment by desired bacterial species but also indicate specific functions of individual gut microbiota members.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microorganisms

ISSN

2076-2607

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

496

UT code for WoS article

000502273600019

EID of the result in the Scopus database

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