First detection and characterization of porcine hemagglutinating encephalomyelitis virus in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000340" target="_blank" >RIV/00027162:_____/19:N0000340 - isvavai.cz</a>

Result on the web

<a href="https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=95_2018-VETMED" target="_blank" >https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=95_2018-VETMED</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/95/2018-VETMED" target="_blank" >10.17221/95/2018-VETMED</a>

Result language

čeština

Original language name

První průkaz a charakterizace viru porcinní hemaglutinující encefalomyelitidy v České republice

Original language description

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system in piglets. The incidence of PHE among pigs in many countries is rising and the economic losses to the pig industry may be significant. According to the serological studies, it is assumed that PHEV is spread worldwide. However, no surveillance has been carried out in the Czech Republic. During this study eight pig farms were screened for the presence of members of Coronaviridae family with the use of reverse transcription PCR. The collection of 123 faecal samples and 151 nasal swabs from domestic pigs were analysed. In PHEV-positive samples almost complete coding sequence of nucleocapsid gene was amplified and the acquired sequences were compared to those of geographically different PHEV strains and phylogenetic analyses was performed. PHEV was present in 7.9% of nasal swabs taken from different age categories of pigs. No other swine coronaviruses were detected. The phylogeny showed 95.8-98.1% similarity in amino acid sequence of the Czech PHEV strains compared to other PHEV reference strains in GenBank. The PHEV strains collected from animals on the same farm were identical; however, strains from different farms have only 96.7-98.7% of amino acid sequence similarity. Our study demonstrated presence of PHEV in pigs in the Czech Republic. The Czech PHEV strains were evolutionary closest to the Belgium strain VW572.

Czech name

První průkaz a charakterizace viru porcinní hemaglutinující encefalomyelitidy v České republice

Czech description

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system in piglets. The incidence of PHE among pigs in many countries is rising and the economic losses to the pig industry may be significant. According to the serological studies, it is assumed that PHEV is spread worldwide. However, no surveillance has been carried out in the Czech Republic. During this study eight pig farms were screened for the presence of members of Coronaviridae family with the use of reverse transcription PCR. The collection of 123 faecal samples and 151 nasal swabs from domestic pigs were analysed. In PHEV-positive samples almost complete coding sequence of nucleocapsid gene was amplified and the acquired sequences were compared to those of geographically different PHEV strains and phylogenetic analyses was performed. PHEV was present in 7.9% of nasal swabs taken from different age categories of pigs. No other swine coronaviruses were detected. The phylogeny showed 95.8-98.1% similarity in amino acid sequence of the Czech PHEV strains compared to other PHEV reference strains in GenBank. The PHEV strains collected from animals on the same farm were identical; however, strains from different farms have only 96.7-98.7% of amino acid sequence similarity. Our study demonstrated presence of PHEV in pigs in the Czech Republic. The Czech PHEV strains were evolutionary closest to the Belgium strain VW572.

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

1805-9392

Volume of the periodical

64

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

60-66

UT code for WoS article

000459023400002

EID of the result in the Scopus database

2-s2.0-85063731253