Subchronic continuous inhalation exposure to zinc oxide nanoparticles induces pulmonary cell response in mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000116" target="_blank" >RIV/00027162:_____/20:N0000116 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/20:00525577 RIV/67985858:_____/20:00525577 RIV/67985904:_____/20:00525577 RIV/00216224:14310/20:00116178

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0946672X20300766" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0946672X20300766</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtemb.2020.126511" target="_blank" >10.1016/j.jtemb.2020.126511</a>

Result language

angličtina

Original language name

Subchronic continuous inhalation exposure to zinc oxide nanoparticles induces pulmonary cell response in mice

Original language description

Objectives: We used mice as an animal model to investigate the entry of ZnO nanoparticles from the ambient air into the lungs and other organs, subsequent changes in Zn levels and the impact on the transcription of Zn homeostasis-related genes in the lungs. Methods: The mice were exposed to two concentrations of ZnO nanoparticles; lower (6.46 x 10(4) particles/cm(3)) and higher (1.93 x 10(6) particles/cm(3)), allowed to breathe the nanoparticles in the air for 12 weeks and subjected to necropsy. Characterization of the ZnO nanoparticles was done using transmission electron microscopy (TEM). Energy-dispersive X-ray (EDX) spectroscopy was used to quantify ZnO nanoparticles in the lungs, brain, liver and kidney. The total zinc content in the lungs, brain, liver, kidney, red blood cells and plasma was estimated by inductively coupled plasma mass spectroscopy (ICP-MS). Transcription rate of the genes was evaluated by RealTime PCR. Results: The two concentration of ZnO nanoparticles in the ambient air produced two different outcomes. The lower concentration resulted in significant increases in Zn content of the liver while the higher concentration significantly increased Zn in the lungs (p < 0.05). Additionally, at the lower concentration, Zn content was found to be lower in brain tissue (p < 0.05). Using TEM/EDX we detected ZnO nanoparticles inside the cells in the lungs, kidney and liver. Inhaling ZnO NP at the higher concentration increased the levels of mRNA of the following genes in the lungs: Mt2 (2.56 fold), Slc30a1 (1.52 fold) and S1c30a5 (2.34 fold). At the lower ZnO nanoparticle concentration, only S1c30a7 mRNA levels in the lungs were up (1.74 fold). Thus the two air concentrations of ZnO nanoparticles produced distinct effects on the expression of the Zn-homeostasis related genes.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21001 - Nano-materials (production and properties)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY

ISSN

0946-672X

e-ISSN

—

Volume of the periodical

61

Issue of the periodical within the volume

Sep 2020

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

"126511"

UT code for WoS article

000543366700011

EID of the result in the Scopus database

2-s2.0-85083016978