Soil protein as a potential antimicrobial agent against methicillin–resistant Staphylococcus aureus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000129" target="_blank" >RIV/00027162:_____/20:N0000129 - isvavai.cz</a>
Alternative codes found
RIV/86652079:_____/20:00525401 RIV/62156489:43210/20:43918043 RIV/00216305:26620/20:PU137242
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0013935120302139?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013935120302139?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.envres.2020.109320" target="_blank" >10.1016/j.envres.2020.109320</a>
Alternative languages
Result language
angličtina
Original language name
Soil protein as a potential antimicrobial agent against methicillin–resistant Staphylococcus aureus
Original language description
Recently, the interest is increasing to find alternatives to replace the usage of antibiotics since their massive and improper usage enhance the antibiotic resistance in human pathogens. In this study, for the first time we showed that the soil proteins have very high antibacterial activity (98% of growth inhibition) against methicillin resistant Staphylococcus aureus (MRSA), one of the most threatening human pathogens. We found that the protein extract (C3) from the forest with past intensive management showed higher antibacterial activity than that of unmanaged forest. The MIC and IC50 were found to be 30 and 15.0 μg protein g−1 dry soil respectively. C3 was found to kill the bacteria by cell wall disruption and genotoxicity which was confirmed by optical and fluorescent microscopy and comet assay. According to qPCR study, the mecA (the antibiotic resistant gene) expression in MRSA was found to be down-regulated after C3 treatment. In contrast, C3 showed no hemolytic toxicity on human red blood cells which was confirmed by hemolytic assay. According to ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS), 144 proteins were identified in C3 among which the majority belonged to Gram negative bacteria (45.8%). Altogether, our results will help to develop novel, costeffective, non-toxic and highly efficient antibacterial medicines from natural sources against antibiotic resistant infections.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ENVIRONMENTAL RESEARCH
ISSN
0013-9351
e-ISSN
1096-0953
Volume of the periodical
188
Issue of the periodical within the volume
SEP 2020
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
"109320"
UT code for WoS article
000564662200021
EID of the result in the Scopus database
2-s2.0-85086369098