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Development and inter-laboratory validation of diagnostics panel for detection of biothreat bacteria based on MOL-PCR assay

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000001" target="_blank" >RIV/00027162:_____/21:N0000001 - isvavai.cz</a>

  • Alternative codes found

    RIV/60162694:G32__/21:N0000002 RIV/60162694:G33__/20:N0000007 RIV/62157124:16270/21:43879157 RIV/60162694:_____/21:N0000010 RIV/00216224:14310/21:00120920

  • Result on the web

    <a href="https://www.mdpi.com/2076-2607/9/1/38" target="_blank" >https://www.mdpi.com/2076-2607/9/1/38</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3390/microorganisms9010038" target="_blank" >10.3390/microorganisms9010038</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Development and inter-laboratory validation of diagnostics panel for detection of biothreat bacteria based on MOL-PCR assay

  • Original language description

    Early detection of biohazardous bacteria that can be misused as biological weapons is one of the most important measures to prevent the spread and outbreak of biological warfare. The purpose of this study was to establish a new detection panel for biothreat bacteria (Bacillus anthracis, Yersinia pestis, Francisella tularensis and Brucella spp.) and confirm it by collaborative validation by using a multiplex oligonucleotide ligation followed by polymerase chain reaction and hybridization to microspheres by MagPix detection platform (MOL-PCR). The specific sequences in bacterial DNA were selected and MOLigo pair probes were designed. No cross-reactivity was recorded during testing between biothreat bacteria or using other selected unrelated bacterial agents. The sensitivities of these assays were at least 0.5 ng/µL for all target bacteria when serially diluted DNA was used as a template. When testing the freezing stability of ligation premixes, it was better to store MOLigo probes separately as probes 1 and probes 2. This highly multiplex method can be used for rapid screening to prevent outbreaks arising from the use of bacterial strains for bioterrorism.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Microorganism

  • ISSN

    2076-2607

  • e-ISSN

    2076-2607

  • Volume of the periodical

    9

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    15

  • Pages from-to

    "38"

  • UT code for WoS article

    000610579000001

  • EID of the result in the Scopus database

    2-s2.0-85098765871