Development and inter-laboratory validation of diagnostics panel for detection of biothreat bacteria based on MOL-PCR assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000001" target="_blank" >RIV/00027162:_____/21:N0000001 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G32__/21:N0000002 RIV/60162694:G33__/20:N0000007 RIV/62157124:16270/21:43879157 RIV/60162694:_____/21:N0000010 RIV/00216224:14310/21:00120920
Result on the web
<a href="https://www.mdpi.com/2076-2607/9/1/38" target="_blank" >https://www.mdpi.com/2076-2607/9/1/38</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/microorganisms9010038" target="_blank" >10.3390/microorganisms9010038</a>
Alternative languages
Result language
angličtina
Original language name
Development and inter-laboratory validation of diagnostics panel for detection of biothreat bacteria based on MOL-PCR assay
Original language description
Early detection of biohazardous bacteria that can be misused as biological weapons is one of the most important measures to prevent the spread and outbreak of biological warfare. The purpose of this study was to establish a new detection panel for biothreat bacteria (Bacillus anthracis, Yersinia pestis, Francisella tularensis and Brucella spp.) and confirm it by collaborative validation by using a multiplex oligonucleotide ligation followed by polymerase chain reaction and hybridization to microspheres by MagPix detection platform (MOL-PCR). The specific sequences in bacterial DNA were selected and MOLigo pair probes were designed. No cross-reactivity was recorded during testing between biothreat bacteria or using other selected unrelated bacterial agents. The sensitivities of these assays were at least 0.5 ng/µL for all target bacteria when serially diluted DNA was used as a template. When testing the freezing stability of ligation premixes, it was better to store MOLigo probes separately as probes 1 and probes 2. This highly multiplex method can be used for rapid screening to prevent outbreaks arising from the use of bacterial strains for bioterrorism.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microorganism
ISSN
2076-2607
e-ISSN
2076-2607
Volume of the periodical
9
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
"38"
UT code for WoS article
000610579000001
EID of the result in the Scopus database
2-s2.0-85098765871