Detection of Mycobacterium avium subspecies paratuberculosis in environmental samples from infected Czech dairy herds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000002" target="_blank" >RIV/00027162:_____/21:N0000002 - isvavai.cz</a>
Result on the web
<a href="https://www.agriculturejournals.cz/publicFiles/168_2020-VETMED.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/168_2020-VETMED.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/168/2020-VETMED" target="_blank" >10.17221/168/2020-VETMED</a>
Alternative languages
Result language
angličtina
Original language name
Detection of Mycobacterium avium subspecies paratuberculosis in environmental samples from infected Czech dairy herds
Original language description
The objective of the present study was to evaluate suitability of environmental sampling for screening Czech dairy herds to detect Mycobacterium avium ssp. paratuberculosis (MAP) and to find the most convenient location for MAP detection in the lactating cow area. Environmental samples (ES, n = 72) from milking parlour holding pen (n = 19), milking alleyways (n = 19) and free-stall alleyways (n = 34) from 19 herds were simultaneously tested to detect MAP by quantitative PCR (qPCR) and bacterial culture. Eight and 13 samples from milking parlour holding pens, 12 and 11 samples from milking alleyways and 11 and 18 samples from free-stall alleyways were qPCR and culture positive, respectively. The 4.6 times higher probability to be culture than qPCR positive was detected for assessable MAP detection results from free-stall alleyways (P-value 0.0086, OR 4.5728) and no association was found between the results from milking parlour holding pens (P-value 0.1914) and milking alleyways (P-value >0.9999) and the diagnostic method used. The percentage of qPCR-positive samples in the tested locations was detected for milking alleyways (63.2%), free-stall alleyways and milking parlour holding pen. The herd infectious status was in agreement with 16 (84.2%), 14 (73.7%) and 12 (63.2%) qPCR results from milking alleyways, free-stall alleyways and milking parlour holding pen, respectively. No statistically significant differences were detected for these results (P-value 0.3961). MAP was detected by qPCR and culture in all three locations where ES were collected. We suggested environmental sampling followed by MAP detection by qPCR as an easy-to-do time-saving protocol for MAP screening in Czech dairy herds. Although the milking alleyways seem to be the most convenient location for environmental sampling, this assumption was not statistically supported.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/QK1820086" target="_blank" >QK1820086: Paratuberculosis – prevalence and control programme in cattle herds</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Veterinární medicína
ISSN
0375-8427
e-ISSN
1805-9392
Volume of the periodical
66
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
1-7
UT code for WoS article
000606544600001
EID of the result in the Scopus database
2-s2.0-85099110543