The colonisation of Czech travellers and expatriates living in the Czech Republic by colistin-resistant Enterobacteriaceae and whole genome characterisation of E. coli isolates harbouring the mcr-1 genes on a plasmid or chromosome: A cross-sectional study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000010" target="_blank" >RIV/00027162:_____/21:N0000010 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/21:10417651 RIV/00064211:_____/21:W0000042 RIV/00064203:_____/21:10417651 RIV/00216224:14310/21:00120823
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1477893920304105?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1477893920304105?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tmaid.2020.101914" target="_blank" >10.1016/j.tmaid.2020.101914</a>
Alternative languages
Result language
angličtina
Original language name
The colonisation of Czech travellers and expatriates living in the Czech Republic by colistin-resistant Enterobacteriaceae and whole genome characterisation of E. coli isolates harbouring the mcr-1 genes on a plasmid or chromosome: A cross-sectional study
Original language description
Background: Travellers were recognized as risk cohort that can be colonized by mcr-1-mediated colistin resistant Enterobacteriaceae. We aimed to investigate the carriage of mcr-mediated colistin resistance in Enterobacteriaceae in Czech travellers or expatriates residing temporarily in the Czech Republic. Methods: Between August 2018 and September 2019, the stool samples were cultured in Enterobacteriaceae enrichment broth. The enriched cultures were tested for the presence of mcr-1-8 genes and inoculated onto selective agar with colistin. A minimal inhibitory concentration of colistin was determined by using the broth microdilution method. Colistin resistant Enterobacteriaceae isolates were tested for the presence of mcr-1-8 genes and mcr-positive isolates were characterised by whole genome sequencing. Results: From 177 stool samples, 15 Enterobacteriaceae isolates were cultured (7.9%); two E. coli isolates carried mcr-1 gene (1.1%). In E. coli sequence type (ST) 156, the mcr-1 gene was located in an ISApl1-mcr-1-orf-ISApl1 (Tn6330) and incorporated into the chromosome; in E. coli ST23 isolate, the mcr-1 gene was harboured by the plasmid IncX4. Both, mcr-1 positive E. coli were multidrug resistant and, in addition, one isolate was an extended-spectrum β-lactamase (ESBL) producer (blaCTX-M-27). Conclusion: Czech travellers, or expatriates in the Czech Republic, can be colonized by multidrug resistant Enterobacteriaceae harbouring the mcr-1 gene.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
<a href="/en/project/NV18-09-00254" target="_blank" >NV18-09-00254: Sentinel testing survey of plasmid-mediated (transferable) colistin resistance among Enterobacteriaceae in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Travel Medicine and Infectious Disease
ISSN
1477-8939
e-ISSN
1873-0442
Volume of the periodical
39
Issue of the periodical within the volume
JAN 2021
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
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UT code for WoS article
000620092200007
EID of the result in the Scopus database
2-s2.0-85096467826