Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000076" target="_blank" >RIV/00027162:_____/21:N0000076 - isvavai.cz</a>
Result on the web
<a href="https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=190_2020-VETMED" target="_blank" >https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=190_2020-VETMED</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/190/2020-VETMED" target="_blank" >10.17221/190/2020-VETMED</a>
Alternative languages
Result language
angličtina
Original language name
Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens
Original language description
A rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics properties of trimethoprim and sulfamethoxazole were investigated in clinically healthy broiler chickens after the single oral administration of the commercial preparation Methoxasol (Eurovet Animal Health, B.V., The Netherlands) at a dose of 0.275 ml/kg b.w. After a single dose drug administration, the chickens were sacrificed by decapitation under general anaesthesia by Isoflurin 1 000 mg/g (Vetpharma AH, Spain) and the blood was collected at precisely defined intervals: 15, 30, 45, 60, 90, 120, 180, 360 and 720 min after the administration. The serum concentrations of amoxicillin were determined using Q Exactive tandem mass spectrometer (Thermo Fisher Scientific, USA) in conjunction with liquid chromatography. The detected pharmacokinetic parameters of trimethoprim after the oral administration were Cmax = 2.1 ± 1.0 µg/ml; Tmax = 1.5 h; t½ = 0.88 h; kel = 0.009 3 ± 0.001 1 1/h; AUCt = 2.901 ± 1.4 µg.h/ml; AUC∞ = 2.907 ± 1.5 µg.h/ml; Vd = 2.632 l/kg; Cl = 2.7 l/h. The pharmacokinetic parameters of sulfamethoxazole after the oral administration were Cmax = 47.1 ± 15.3 µg/ml; Tmax = 1 h; t½ = 1.92 h; kel = 0.004 6 ± 0.000 3 1/h; AUCt = 89.676 ± 26.9 µg.h/ml; AUC∞ = 94.612 ± 28.4 µg.h/ml; Vd = 0.584 l/kg; Cl = 0.21 l/h. To the best of our knowledge, this is the first pharmacokinetic study of the combination of sulfamethoxazole and trimethoprim in broiler chickens.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/QK1910057" target="_blank" >QK1910057: Genetaring of the data for determination of maximal residual limit for clavulanic acid in edible tissues of domestic fowl and pharmacokinetic and pharmacodynamic parameters of selected antimicrobials in broiler chickens</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Veterinární medicína
ISSN
0375-8427
e-ISSN
1805-9392
Volume of the periodical
66
Issue of the periodical within the volume
6
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
248-256
UT code for WoS article
000657258700003
EID of the result in the Scopus database
2-s2.0-85107462592