Optimisation of phagocytosis assay in rainbow trout (Oncorhynchus mykiss)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000081" target="_blank" >RIV/00027162:_____/21:N0000081 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/21:43879782 RIV/62156489:43210/21:43919939
Result on the web
<a href="https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=189_2020-VETMED" target="_blank" >https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=189_2020-VETMED</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/189/2020-VETMED" target="_blank" >10.17221/189/2020-VETMED</a>
Alternative languages
Result language
angličtina
Original language name
Optimisation of phagocytosis assay in rainbow trout (Oncorhynchus mykiss)
Original language description
A phagocytosis assay is one of the most commonly used functional immunological methods. There are many possible ways of assessing leucocytes and their ability to ingest different particles. The aim of this study was to optimise the phagocytosis assay in rainbow trout (Oncorhynchus mykiss) using labelled zymosan particles (Alexa Fluor 488 and Texas Red conjugate). Whole blood was incubated with the particles under different conditions and leucocytes were subsequently isolated by haemolysis in a hypotonic environment. The effect of the different incubation time, temperature, blood volume and dilution on the phagocytic activity was evaluated by flow cytometry. Our experiments showed that the incubation for at least 2 h at 15 °C provided optimal results, while the blood volume and dilution had no significant effect. The optimised assay will be used for the examination of fish health and in further experimental studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Veterinární medicína
ISSN
0375-8427
e-ISSN
1805-9392
Volume of the periodical
66
Issue of the periodical within the volume
7
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
298-304
UT code for WoS article
000657259300004
EID of the result in the Scopus database
2-s2.0-85107493941