Comparison of diagnostic methods for Tetracapsuloides bryosalmonae detection in salmonid fish
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000100" target="_blank" >RIV/00027162:_____/21:N0000100 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/21:00541652 RIV/62156489:43210/21:43919671 RIV/62157124:16270/21:43879784
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/jfd.13375" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/jfd.13375</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jfd.13375" target="_blank" >10.1111/jfd.13375</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of diagnostic methods for Tetracapsuloides bryosalmonae detection in salmonid fish
Original language description
Diagnostic accuracy of pathogen detection depends upon the selection of suitable tests. Problems can arise when the selected diagnostic test gives false-positive or false-negative results, which can affect control measures, with consequences for the population health. The aim of this study was to compare sensitivity of different diagnostic methods IHC, PCR and qPCR detecting Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonid fish and as a consequence differences in disease prevalence. We analysed tissue from 388 salmonid specimens sampled from a recirculating system and rivers in the Czech Republic. Overall prevalence of T. bryosalmonae was extremely high at 92.0%, based on positive results of at least one of the above-mentioned screening methods. IHC resulted in a much lower detection rate (30.2%) than both PCR methods (qPCR32: 65.4%, PCR: 81.9%). While qPCR32 produced a good match with IHC (60.8%), all other methods differed significantly (p < .001) in the proportion of samples determined positive. Both PCR methods showed similar sensitivity, though specificity (i.e., the proportion of non-diseased fish classified correctly) differed significantly (p < .05). Sample preservation method significantly (p < .05) influenced the results of PCR, with a much lower DNA yield extracted from paraffin-embedded samples. Use of different methods that differ in diagnostic sensitivity and specificity resulted in random and systematic diagnosis errors, illustrating the importance of interpreting the results of each method carefully.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Sustainable production of healthy fish in various aquaculture systems - PROFISH</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Fish Diseases
ISSN
0140-7775
e-ISSN
1365-2761
Volume of the periodical
44
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1147–1153
UT code for WoS article
000638551400001
EID of the result in the Scopus database
2-s2.0-85104125419