Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000132" target="_blank" >RIV/00027162:_____/21:N0000132 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2076-2615/11/7/2106" target="_blank" >https://www.mdpi.com/2076-2615/11/7/2106</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ani11072106" target="_blank" >10.3390/ani11072106</a>
Alternative languages
Result language
angličtina
Original language name
Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa
Original language description
Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animals
ISSN
2076-2615
e-ISSN
2076-2615
Volume of the periodical
11
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
5
Pages from-to
"2106"
UT code for WoS article
000675926500001
EID of the result in the Scopus database
2-s2.0-85110229174