RECENT CARP EDEMA VIRUS ISOLATES FROM CZECHIA ARE HIGHLY SIMILAR TO ISOLATES FROM CONTINENTAL EUROPE

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000185" target="_blank" >RIV/00027162:_____/21:N0000185 - isvavai.cz</a>

Result on the web

<a href="https://eposters.blob.core.windows.net/eas-eposters/AE2021AbstractBook.pdf" target="_blank" >https://eposters.blob.core.windows.net/eas-eposters/AE2021AbstractBook.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

RECENT CARP EDEMA VIRUS ISOLATES FROM CZECHIA ARE HIGHLY SIMILAR TO ISOLATES FROM CONTINENTAL EUROPE

Original language description

With a stable annual production of around 20 000 tonnes, common carp (Cyprinus carpio) is the most valuable aquaculture species in the Czech Republic. Emerging diseases, such as the carp edema virus disease (CEVD) detected in the country since 2013 (Matejickova et al. 2020) pose a threat to the industry. Molecular epidemiology, in the form of sequencing of isolates, can help understand the distribution of the virus in the country.Material and Methods: In total, 637 samples representing 124 locations (individual farms or ponds) were examined in the year 2020 for the presence of the virus using conventional and real-time PCR (Way et al, 2017). Partial P4a gene sequences (357 bp) of detected CEV isolates were obtained via Sanger sequencing and compared to isolated uploaded to the GenBank database. Results: CEV was diagnosed in six locations, namely four common carp farms and two koi carp ponds. Clinical signs in farmed common carp varied from asymptomatic to a 100% mortality rate in one of the facilities, meanwhile both positive koi in ponds showed sleepy-disease-like symptoms. Phylogenetically, two of the established CEV genogroups were detected: Genogroup I in common carp and genogroup IIa in koi. The isolates from common carp showed up to 100% identity with previously published sequences from Czechia, Germany, Hungary and Great Britain. Conclusions: High levels of similarity in the routinely sequenced partial P4a gene of carp edema virus suggest wide dissemination of the pathogen and possible transmission of specific isolates, most probably via the transport of live fish, ornamental or farmed.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů