Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000249" target="_blank" >RIV/00027162:_____/22:N0000249 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43905357 RIV/60077344:_____/22:00571902 RIV/00216224:14310/22:00128502
Result on the web
<a href="https://www.mdpi.com/1999-4915/14/12/2673" target="_blank" >https://www.mdpi.com/1999-4915/14/12/2673</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/v14122673" target="_blank" >10.3390/v14122673</a>
Alternative languages
Result language
angličtina
Original language name
Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models
Original language description
The tick-borne encephalitis virus (TBEV) causes a most important viral life-threatening illness transmitted by ticks. The interactions between the virus and ticks are largely unexplored, indicating a lack of experimental tools and systematic studies. One such tool is recombinant reporter TBEV, offering antibody-free visualization to facilitate studies of transmission and interactions between a tick vector and a virus. In this paper, we utilized a recently developed recombinant TBEV expressing the reporter gene mCherry to study its fitness in various tick-derived in vitro cell cultures and live unfed nymphal Ixodes ricinus ticks. The reporter virus was successfully replicated in tick cell lines and live ticks as confirmed by the plaque assay and the mCherry-specific polymerase chain reaction (PCR). Although a strong mCherry signal determined by fluorescence microscopy was detected in several tick cell lines, the fluorescence of the reporter was not observed in the live ticks, corroborated also by immunoblotting. Our data indicate that the mCherry reporter TBEV might be an excellent tool for studying TBEV-tick interactions using a tick in vitro model. However, physiological attributes of a live tick, likely contributing to the inactivity of the reporter, warrant further development of reporter-tagged viruses to study TBEV in ticks in vivo.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Viruses
ISSN
1999-4915
e-ISSN
1999-4915
Volume of the periodical
14
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
"2673"
UT code for WoS article
000904310800001
EID of the result in the Scopus database
2-s2.0-85144505223