Supernumerary Marker Chromosome Identified in Asian Elephant (Elephas maximus)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000016" target="_blank" >RIV/00027162:_____/23:N0000016 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2076-2615/13/4/701" target="_blank" >https://www.mdpi.com/2076-2615/13/4/701</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ani13040701" target="_blank" >10.3390/ani13040701</a>
Alternative languages
Result language
angličtina
Original language name
Supernumerary Marker Chromosome Identified in Asian Elephant (Elephas maximus)
Original language description
We identified a small, supernumerary marker chromosome (sSMC) in two phenotypically normal Asian elephants (Elephas maximus): a female (2n = 57,XX,+mar) and her male offspring (2n = 57,XY,+mar). sSMCs are defined as structurally abnormal chromosomes that cannot be identified by conventional banding analysis since they are usually small and often lack distinct banding patterns. Although current molecular techniques can reveal their origin, the mechanism of their formation is not yet fully understood. We determined the origin of the marker using a suite of conventional and molecular cytogenetic approaches that included (a) G- and C-banding, (b) AgNOR staining, (c) preparation of a DNA clone using laser microdissection of the marker chromosome, (d) FISH with commercially available human painting and telomeric probes, and (e) FISH with centromeric DNA derived from the centromeric regions of a marker-free Asian elephant. Moreover, we present new information on the location and number of NORs in Asian and savanna elephants. We show that the metacentric marker was composed of heterochromatin with NORs at the terminal ends, originating most likely from the heterochromatic region of chromosome 27. In this context, we discuss the possible mechanism of marker formation. We also discuss the similarities between sSMCs and B chromosomes and whether the marker chromosome presented here could evolve into a B chromosome in the future.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animals
ISSN
2076-2615
e-ISSN
2076-2615
Volume of the periodical
13
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
"701"
UT code for WoS article
000937863100001
EID of the result in the Scopus database
2-s2.0-85148854045