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Staphylococcus aureus Collagen-based Wound Dressings enriched with Thermostable Enzybiotics

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000066" target="_blank" >RIV/00027162:_____/23:N0000066 - isvavai.cz</a>

  • Result on the web

    <a href="https://ubch.sci.muni.cz/pro-studenty/konference-doktorandu#abstracts" target="_blank" >https://ubch.sci.muni.cz/pro-studenty/konference-doktorandu#abstracts</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Staphylococcus aureus Collagen-based Wound Dressings enriched with Thermostable Enzybiotics

  • Original language description

    Staphylococci, especially Staphylococcus aureus, are the most common cause of skin and soft tissue infections. Lytic enzymes originating from bacteria or bacteriophages represent alternative therapeutics for bacterial infection. The antibacterial effect results from their degrading activity towards bacterial peptidoglycan. Biopolymers, in addition to their function as a physical barrier and absorptive material, stimulate the wound healing process and can be used as a scaffold for therapeutics. We tested the antimicrobial activity of two recombinantly modified enzymes derived from lysostaphin (LYSSTAPH-S) and endolysin (LYSDERM-S). Thermostable mutations were introduced into the genetic information of each enzyme using seamless cloning. An increase in the thermal stability was verified by a thermal shift assay. Selected enzymes were incorporated in CMC/collagen or water-soluble foams. Antimicrobial susceptibility testing was performed by plate lysis and turbidity assay against MRSA strains. Our thermostable enzybiotics in wound dressing showed antimicrobial effect against MRSA pathogens and pointed out their potential for the treatment of staphylococcal wound infection.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/NU22-05-00475" target="_blank" >NU22-05-00475: Treatment of Infections Caused by Multidrug-Resistant Bacterial Strains by Novel Antibacterial Preparations Based on Cathelicidin Antimicrobial Peptides</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů