Staphylococcus aureus Collagen-based Wound Dressings enriched with Thermostable Enzybiotics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000066" target="_blank" >RIV/00027162:_____/23:N0000066 - isvavai.cz</a>
Result on the web
<a href="https://ubch.sci.muni.cz/pro-studenty/konference-doktorandu#abstracts" target="_blank" >https://ubch.sci.muni.cz/pro-studenty/konference-doktorandu#abstracts</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Staphylococcus aureus Collagen-based Wound Dressings enriched with Thermostable Enzybiotics
Original language description
Staphylococci, especially Staphylococcus aureus, are the most common cause of skin and soft tissue infections. Lytic enzymes originating from bacteria or bacteriophages represent alternative therapeutics for bacterial infection. The antibacterial effect results from their degrading activity towards bacterial peptidoglycan. Biopolymers, in addition to their function as a physical barrier and absorptive material, stimulate the wound healing process and can be used as a scaffold for therapeutics. We tested the antimicrobial activity of two recombinantly modified enzymes derived from lysostaphin (LYSSTAPH-S) and endolysin (LYSDERM-S). Thermostable mutations were introduced into the genetic information of each enzyme using seamless cloning. An increase in the thermal stability was verified by a thermal shift assay. Selected enzymes were incorporated in CMC/collagen or water-soluble foams. Antimicrobial susceptibility testing was performed by plate lysis and turbidity assay against MRSA strains. Our thermostable enzybiotics in wound dressing showed antimicrobial effect against MRSA pathogens and pointed out their potential for the treatment of staphylococcal wound infection.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NU22-05-00475" target="_blank" >NU22-05-00475: Treatment of Infections Caused by Multidrug-Resistant Bacterial Strains by Novel Antibacterial Preparations Based on Cathelicidin Antimicrobial Peptides</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů