A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000076" target="_blank" >RIV/00027162:_____/23:N0000076 - isvavai.cz</a>
Result on the web
<a href="https://journals.pan.pl/dlibra/publication/145024/edition/127674/content" target="_blank" >https://journals.pan.pl/dlibra/publication/145024/edition/127674/content</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.24425/pjvs.2023.145024" target="_blank" >10.24425/pjvs.2023.145024</a>
Alternative languages
Result language
angličtina
Original language name
A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR
Original language description
Cultivation-based assays represent the gold standard for the assessment of the virus infectivity; however, they are time-consuming and not suitable for each virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. Present study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, with the attention to two significant pathogens of livestock – bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl2) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl2-COD) produced the highest differences found between native and heat-treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 minutes, 4 °C) were applied on the ASFV and the heat inactivation profile of ASFV was assessed. Significant decrease of detected quantity of ASFV DNA after heat-treatment (60 °C and 95 °C) and consequent incubation with Pd compounds was observed. BB-PdCl2 and PdCl2-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses like BoHV-1 or ASFV.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/QK1920113" target="_blank" >QK1920113: African swine fever virus in meat and products thereof – detection methods and persistence studies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Polish Journal of Veterinary Science
ISSN
1505-1773
e-ISSN
2300-2557
Volume of the periodical
26
Issue of the periodical within the volume
2
Country of publishing house
PL - POLAND
Number of pages
11
Pages from-to
211-221
UT code for WoS article
001023483300005
EID of the result in the Scopus database
2-s2.0-85164230067