Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000140" target="_blank" >RIV/00027162:_____/23:N0000140 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/23:43880852

Result on the web

<a href="https://sciendo.com/article/10.2478/jvetres-2023-0046" target="_blank" >https://sciendo.com/article/10.2478/jvetres-2023-0046</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/jvetres-2023-0046" target="_blank" >10.2478/jvetres-2023-0046</a>

Result language

angličtina

Original language name

Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies?

Original language description

Introduction: Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (Apismellifera L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey beevirus detection. Material and Methods: Honey, pollen, hive debris, hive grid smears and forager honey bees were collectedfrom 24 hives at four locations in the Czech Republic. Deformed wing virus (DWV), acute bee paralysis virus (ABPV), sacbroodvirus (SBV) and black queen cell virus (BQCV) were detected using a reverse transcription PCR (RT-PCR) and real-timequantitative RT-PCR and the results for bees and alternative materials compared. Results: All forager bee samples containedDWV, BQCV and SBV and 54.2% had ABPV. When comparing beehive materials to bees, the most promising results wereobtained from honey and pollen samples, with BQCV and SBV detected in all honey samples and ABPV in 12.5%. Detection ofSBV was achieved in 91.6% of pollen samples, detection of BQCV in 87.5% and detection of DWW in 75%. The results fordebris and smears were less consistent with the viral profile of the forager samples. Conclusion: The best candidate materials forhoney bee virus detection in a non-invasive technique are honey and pollen.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10607 - Virology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Veterinary Research

ISSN

2450-7393

e-ISSN

2450-8608

Volume of the periodical

67

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

323-331

UT code for WoS article

001065223400001

EID of the result in the Scopus database

2-s2.0-85173547728