UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000166" target="_blank" >RIV/00027162:_____/23:N0000166 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10477443
Result on the web
<a href="https://www.nature.com/articles/s41598-023-46791-9" target="_blank" >https://www.nature.com/articles/s41598-023-46791-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-023-46791-9" target="_blank" >10.1038/s41598-023-46791-9</a>
Alternative languages
Result language
angličtina
Original language name
UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine
Original language description
Ultraviolet irradiation is an efective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fuorescent GFP protein produced by baculovirus expression system after the irradiation of baculovirus culture in doses ranging from 3.5 to 42 J/m2 was determined. At a dose of 36.8 J/m2 , only 0.5% of GFP-expressing cells were detected by fow cytometry and confocal microscopy. The stability of purifed VP1-PCV2bCap protein produced by baculovirus expression system was analyzed after the irradiation at doses ranging from 3.5 to 19.3 J/m2 . Up to the dose of 11 J/m2 , no signifcant efect of UV-C light on the stability of VP1PCV2bCap was detected. We observed a dose-dependent increase in VP1-PCV2bCap-specifc immune response in BALB/c mice immunized by recombinant protein sterilized by irradiation in dose 11 J/m2 with no signifcant diference between vaccines sterilized by UV-C light and fltration. A substantial diference in the production of VP1-PCV2bCap specifc IgG was observed in piglets immunized with VP1-PCV2bCap sterilized by UV-C in comparison with protein sterilized by fltration in combination with the inactivation of baculovirus by binary ethylenimine. UV-C irradiation represents an efective method for vaccine sterilization, where commonly used methods of sterilization are not possible.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/EF16_025%2F0007397" target="_blank" >EF16_025/0007397: Recombinant Biotechnology and Immunotherapy Centre</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
13
Issue of the periodical within the volume
2023
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
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UT code for WoS article
001101369900095
EID of the result in the Scopus database
2-s2.0-85175966726