Cytogenetic Analysis of the Fish Genus Carassius Indicates Divergence, Fission, and Segmental Duplication as Drivers of Tandem Repeat and Microchromosome Evolution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000024" target="_blank" >RIV/00027162:_____/24:N0000024 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10479027

Result on the web

<a href="https://academic.oup.com/gbe/article/16/3/evae028/7606233" target="_blank" >https://academic.oup.com/gbe/article/16/3/evae028/7606233</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/gbe/evae028" target="_blank" >10.1093/gbe/evae028</a>

Result language

angličtina

Original language name

Cytogenetic Analysis of the Fish Genus Carassius Indicates Divergence, Fission, and Segmental Duplication as Drivers of Tandem Repeat and Microchromosome Evolution

Original language description

Fishes of the genus Carassius are useful experimental vertebrate models for study of evolutionary biology and cytogenetics. Carassius demonstrates diverse biological characteristics, such as variation in ploidy levels and chromosome numbers, and presence of microchromosomes. Carassius polyploids with ≥ 150 chromosomes have microchromosomes, but their origin, especially in European populations, is unknown. We used cytogenetics to study evolution of tandem repeats (U1 and U2 small nuclear DNAs and H3 histone) and microchromosomes in Carassius from the Czech Republic. We tested the hypotheses whether the number of tandem repeats was affected by polyploidization or divergence between species and what mechanism drives evolution of microchromosomes. Tandem repeats were found in tetraploid and hexaploid C. gibelio, tetraploid C. auratus and C. carassius in conserved numbers, with the exception of U1 snDNA in C. auratus. This conservation indicates reduction and/or loss in the number of copies per locus that may have occurred by divergence rather than polyploidization. To study evolution of microchromosomes, we used the whole microchromosome painting probe from hexaploid C. gibelio and hybridized it to tetraploid and hexaploid C. gibelio, tetraploid C. auratus and C. carassius. Our results revealed variation in the number of microchromosomes in hexaploids, indicating that the evolution of Carassius karyotype is governed by macrochromosome fissions followed by segmental duplication in pericentromeric areas. This is a potential mechanism responsible for the presence of microchromosomes in Carassius hexaploids. Differential efficacy of one or both of these mechanisms in different tetraploids could ensure variability in chromosome number in polyploids in general.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/EH22_010%2F0002902" target="_blank" >EH22_010/0002902: MSCA Fellowships CZ - Charles University</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome Biology and Evolution

ISSN

1759-6653

e-ISSN

1759-6653

Volume of the periodical

16

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

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UT code for WoS article

001176621200001

EID of the result in the Scopus database

2-s2.0-85186696677