A scoring system for histological evaluation of skin during Staphylococcus aureus infections
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000201" target="_blank" >RIV/00027162:_____/24:N0000201 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/23:00079502 RIV/00216224:14110/24:00135303 RIV/61988987:17110/24:A2502O0O RIV/00159816:_____/24:00079502 RIV/00216305:26620/23:PU150708
Result on the web
<a href="https://link.springer.com/article/10.1007/s11756-023-01567-4" target="_blank" >https://link.springer.com/article/10.1007/s11756-023-01567-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11756-023-01567-4" target="_blank" >10.1007/s11756-023-01567-4</a>
Alternative languages
Result language
angličtina
Original language name
A scoring system for histological evaluation of skin during Staphylococcus aureus infections
Original language description
The histological evaluation of skin changes during Staphylococcus aureus (S. aureus) infection requires an exact approach with an accurate definition of individual changes and their parameters, including a classification score facilitating comparison among experiments. To propose methods and procedures for the evaluation of histological changes in skin infected by S. aureus strains in experimental pig models. Four pigs were included in the experiment. One pig was not inoculated and served as control. Subsequently, samples were collected from each wound on Days 3, 7, 10, ad 14 after inoculation processed by frozen section technique and the samples were stained with hematoxylin-eosin. Classification scores of selected parameters were determined and an average classification index was established at the end of the experiment. Histologically, granulomatous arrangement and superficial re-epithelialization were observed in the wounds of all animals. Compared to control, S. aureus infection documents slow healing of skin wounds with impaired epidermal formation, re-epithelialization, oedema in the dermis, inflammation in the dermis, fibrosis in the dermis, fibrosis in the hypodermis and, finally, necrosis in the dermis. The results in the scores for scabs, dermis, and, finally, necrosis in the hypodermis did not differ among groups. The proposed methodological approach allowed quantification of histological changes and we recommend employing this scoring system in other studies evaluating the healing of skin wounds.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biologia
ISSN
0006-3088
e-ISSN
1336-9563
Volume of the periodical
79
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
917-925
UT code for WoS article
001105224900001
EID of the result in the Scopus database
2-s2.0-85176795915