Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000031" target="_blank" >RIV/00027162:_____/25:N0000031 - isvavai.cz</a>

Result on the web

<a href="https://meridian.allenpress.com/jwd/article-abstract/61/2/396/506134/Wildlife-Sentinel-Development-of-Multispecies" target="_blank" >https://meridian.allenpress.com/jwd/article-abstract/61/2/396/506134/Wildlife-Sentinel-Development-of-Multispecies</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7589/JWD-D-24-00028" target="_blank" >10.7589/JWD-D-24-00028</a>

Result language

angličtina

Original language name

Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance

Original language description

Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first emerged in Wuhan in 2019 and rapidly spread worldwide. During the course of the COVID-19 pandemic, numerous reports highlighted infections of wild animals by SARS-CoV-2. Nevertheless, further research is required to understand the virus potential to infect various animal species, which is crucial for evaluating its future evolution and the potential reemergence of SARS-CoV-2. The total concentration of immunoglobulin G (IgG) represents a valuable yet underused diagnostic parameter for health assessments in wild animals, primarily due to the absence of effective diagnostic tools. A protein A-based indirect ELISA can serve as an efficient method to identify IgG antibodies against different pathogens in wildlife surveillance programs. To develop a multispecies protein A-ELISA for IgG detection against SARS-CoV-2, we used 44 animal species serum samples to ascertain the protein A binding affinity, and 88 serum samples, chosen for the strong binding affinity to protein A, were used to identify IgG antibodies against SARS-CoV-2. The serum samples were obtained from animals housed in Safari Park Dvůr Králové, Czech Republic. The zoo animals are in proximity to humans, facilitating the exploration of potential reverse transmission events of SARS-CoV-2 from humans to animals. Also, they undergo routine veterinary examinations, providing convenient access to blood samples. Therefore, they can be easily used for the development of protein A-based indirect ELISA for wildlife disease surveillance programs. Based on the ELISA results, antibodies to SARS-CoV-2 were detected in the sera of 16 animals. To further confirm these findings, the ELISA-positive samples were subjected to virus neutralization assays. This additional testing revealed the presence of SARS-CoV-2 neutralizing antibodies in the serum of two white rhinoceros (Ceratotherium simum simum) and one Persian leopard (Panthera pardus tulliana).

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

<a href="/en/project/NU21-05-00143" target="_blank" >NU21-05-00143: Hidden zoonoses - uncovering new pathogenic agents from wildlife</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Wildlife Diseases

ISSN

0090-3558

e-ISSN

1943-3700

Volume of the periodical

61

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

396–407

UT code for WoS article

999

EID of the result in the Scopus database

2-s2.0-105004897243