Extraintestinal Escherichia coli from camel carcasses: Phylogroups, serotypes, and markers of virulence

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000096" target="_blank" >RIV/00027162:_____/25:N0000096 - isvavai.cz</a>

Result on the web

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0334045" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0334045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0334045" target="_blank" >10.1371/journal.pone.0334045</a>

Result language

angličtina

Original language name

Extraintestinal Escherichia coli from camel carcasses: Phylogroups, serotypes, and markers of virulence

Original language description

Pathogenic Escherichia coli causes infections responsible for economic losses in animal herds worldwide. Although this bacterium is well studied in livestock and poultry, studies of camelid infections caused by extraintestinal pathogenic E. coli (ExPEC) are limited. In this study, a set of ExPEC from camel carcasses (n = 150) was characterized with respect to phylogenetic groups, 162 O serotypes, and 35 virulence-associated genes (VAGs) using PCR screening. ExPEC frequently belonged to phylogroup B1 (58.7%), followed by phylogroups C, A, and B2 (12.7%, 12.0%, and 9.3%, respectively). Additionally, the set of ExPEC contained 36 different serotypes. The ExPEC isolates were found to typically encode ≥5 tested VAGs, particularly those related to adhesion (afaI, fimA, pap, sfa, tsh), iron acquisition (fyuA, iroN, iucC, sitA), host cell damage (α-hly, cdt, cnf1, sat), invasion (ibeA), and bacterial protection (iss, ompT, traT). Moreover, ExPEC from camel adults and calves were different from each other. Among isolates from calves, prevalence was significantly higher for phylogroups C (q < 0.001) and E (q < 0.01), and ten VAGs, including fitness factors (eitA, fepC, fyuA, iroN, iss, iucD, ompT, sitA), as well as VAGs with stronger link to pathogenicity (hlyF, and pap). To identify potential reservoir of camel ExPEC strains, fecal E. coli (n = 139) from healthy camels were also analyzed. Based on the identified characteristics, ExPEC were distinguishable from fecal isolates of healthy camels, suggesting an exogenous source of ExPEC infections, likely transmitted from wild birds and human keepers.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plos One

ISSN

—

e-ISSN

1932-6203

Volume of the periodical

20

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

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UT code for WoS article

001591276000012

EID of the result in the Scopus database

2-s2.0-105018273126