Anti-inflammatory potential of sesquiterpene lactones from Schkuhria pinnata in an in vitro intestinal inflammation model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000198" target="_blank" >RIV/00027162:_____/25:N0000198 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Anti-inflammatory potential of sesquiterpene lactones from Schkuhria pinnata in an in vitro intestinal inflammation model

Original language description

Phytochemical Society of Europe Trends in Natural Product Research 2025 Young Scientists Meeting, Wroclaw, Polsko 2 - 6 June 2025 – poster. Five sesquiterpene lactones were isolated from the above-ground parts of Schkuhria pinnata (Lam.) Kuntze ex Thell., Asteraceae, at the Department of Natural Drugs, Faculty of Pharmacy, Masaryk University. Among these, three compounds were isolated and characterized for the first time. Their potential anti-inflammatory effects were assessed in vitro. Initial screening using the human leukemia monocytic cell line THP1-Blue™ NF-κB, stimulated with lipopolysaccharide (LPS), revealed that two compounds exhibited a greater inhibitory effect on the pro-inflammatory transcription factor NF-κB signaling pathway than the standard drug prednisolone. The reduction in NF-κB activity was further confirmed by decreased mRNA levels of the pro-inflammatory cytokines TNFα and IL-1β in macrophages derived from THP1-Blue™ NF-κB cells. Both compounds were subsequently evaluated in a 3D in vitro model of intestinal inflammation, consisting of a co-culture of differentiated enterocytelike Caco-2 cells and THP-1-derived macrophages in a Transwell® system stimulated with LPS. Within 24 hours, these compounds significantly reduced TNFα mRNA levels in Caco-2 cells. This inhibitory effect was further confirmed via ELISA, which quantified reduced TNFα levels in the basolateral media. These findings highlight the potential of these sesquiterpene lactones as promising anti-inflammatory agents

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů