Urinary Cell-Free DNA Quantification as Non-Invasive Biomarker in Patients with Bladder Cancer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F16%3A10312196" target="_blank" >RIV/00064165:_____/16:10312196 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/16:10312196 RIV/00064203:_____/16:10312196 RIV/00216208:11110/16:10312196

Result on the web

<a href="http://www.karger.com/Article/Pdf/438828" target="_blank" >http://www.karger.com/Article/Pdf/438828</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000438828" target="_blank" >10.1159/000438828</a>

Result language

angličtina

Original language name

Urinary Cell-Free DNA Quantification as Non-Invasive Biomarker in Patients with Bladder Cancer

Original language description

Introduction: Concentration of urinary cell-free DNA (ucfDNA) belongs to potential bladder cancer markers, but the reported results are inconsistent due to the use of various non-standardised methodologies. The aim of the study was to standardise the methodology for ucfDNA quantification as a potential non-invasive tumour biomarker. Material and Methods: In total, 66 patients and 34 controls were enrolled into the study. Volumes of each urine portion (V) were recorded and ucfDNA concentrations (c) were measured using real-time PCR. Total amounts (TA) of ucfDNA were calculated and compared between patients and controls. Diagnostic accuracy of the TA of ucfDNA was determined. Results: The calculation of TA of ucfDNA in the second urine portion was the most appropriate approach to ucfDNA quantification, as there was logarithmic dependence between the volume and the concentration of a urine portion (p = 0.0001). Using this methodology, we were able to discriminate between bladder cancer patients and subjects without bladder tumours (p = 0.0002) with area under the ROC curve of 0.725. Positive and negative predictive value of the test was 90 and 45%, respectively. Conclusion: Quantification of ucf DNA according to our modified method could provide a potential non-invasive biomarker for diagnosis of patients with bladder cancer.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FJ - Surgery including transplantology

OECD FORD branch

—

Project

<a href="/en/project/NT12417" target="_blank" >NT12417: Prospects of MicroRNA, mRNA and FreeDNA for Early Non-invasive Detection and Prognosis Assessment in Urinary Bladder Cancer Patients</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Urologia Internationalis

ISSN

0042-1138

e-ISSN

—

Volume of the periodical

96

Issue of the periodical within the volume

1

Country of publishing house

CH - SWITZERLAND

Number of pages

7

Pages from-to

25-31

UT code for WoS article

000368974200005

EID of the result in the Scopus database

2-s2.0-84956754345