Differentially expressed miRNAs in trisomy 21 placentas
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F16%3A10326060" target="_blank" >RIV/00064165:_____/16:10326060 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/16:10326060
Result on the web
<a href="http://dx.doi.org/10.1002/pd.4861" target="_blank" >http://dx.doi.org/10.1002/pd.4861</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/pd.4861" target="_blank" >10.1002/pd.4861</a>
Alternative languages
Result language
angličtina
Original language name
Differentially expressed miRNAs in trisomy 21 placentas
Original language description
Objective: Molecular pathogenesis of Down syndrome (DS) is still incompletely understood. Epigenetic mechanisms, including miRNAs gene expression regulation, belong to potential influencing factors. The aims of this study were to compare miRNAs expressions in placentas with normal and trisomic karyotype and to associate differentially expressed miRNAs with concrete biological pathways. Methods: A total of 80 CVS samples - 41 with trisomy 21 and 39 with normal karyotype - were included in our study. Results obtained in the pilot study using real-time PCR technology and TaqMan Human miRNA Array Cards were subsequently validated on different samples using individual TaqMan miRNA Assays. Results: Seven miRNAs were verified as upregulated in DS placentas (miR-99a, miR-542-5p, miR-10b, miR-125b, miR-615, let-7c and miR-654); three of these miRNAs are located on chromosome 21 (miR-99a, miR-125b and let-7c). Many essential biological processes, transcriptional regulation or apoptosis, were identified as being potentially influenced by altered miRNA levels. Moreover, miRNAs overexpressed in DS placenta apparently regulate genes involved in placenta development (GJA1, CDH11, EGF, ERVW-1, ERVFRD-1, LEP or INHA). Conclusion: These findings suggest the possible participation of miRNAs in Down syndrome impaired placentation and connected pregnancy pathologies.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Prenatal Diagnosis
ISSN
0197-3851
e-ISSN
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Volume of the periodical
36
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
775-784
UT code for WoS article
000381104800012
EID of the result in the Scopus database
2-s2.0-84981272231