MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F16%3A10327073" target="_blank" >RIV/00064165:_____/16:10327073 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/16:10327073 RIV/00064203:_____/16:10327073 RIV/00216208:11110/16:10327073

Result on the web

<a href="http://dx.doi.org/10.4149/neo_2016_518" target="_blank" >http://dx.doi.org/10.4149/neo_2016_518</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/neo_2016_518" target="_blank" >10.4149/neo_2016_518</a>

Result language

angličtina

Original language name

MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection

Original language description

Urinary bladder carcinoma contributes to 4% of newly diagnosed oncological diseases in the Czech Republic. Biomarkers for its early non-invasive detection are therefore highly desirable. Urine seems to be an ideal source of such biomarkers due to the content of cell-free nucleic acids, especially microRNAs (miRNAs).To find potential biomarkers among miRNAs in urine supernatant, we examined in total 109 individuals (36 controls and 73 bladder cancer patients) in three phases. In the first - discovery - phase, microarray cards with 381 miRNAs were used for miRNA analysis of 13 controls and 46 bladder cancer patients. In the second - verification - phase, the results of this first phase were verified on the same groups of subjects by single-target qPCR assays for the selected miRNAs. For the third - validation - phase, new independent samples of urine supernatant (23 controls and 27 bladder cancer patients) were analyzed using single-target qPCR assays for 13 verified in the previous phase. The results of all phases were normalized to miR-191, miR-28-3p, and miR-200b, which were selected as suitable for our study by the qBase+(R).We found that miR-125b, miR-30b, miR-204, miR-99a, and miR-532-3p are significantly down-regulated in patients' urine supernatant. In our experiments, the analysis of miR-125 levels provided the highest AUC (0.801) with 95.65% specificity and 59.26% sensitivity, the analysis of miR-99a lead to AUC (0.738) with 82.61% specificity and 74.07% sensitivity. We demonstrate that levels of these miRNAs could potentially serve as promising diagnostic markers for the non-invasive diagnostics of bladder cancer.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FJ - Surgery including transplantology

OECD FORD branch

—

Project

<a href="/en/project/NT12417" target="_blank" >NT12417: Prospects of MicroRNA, mRNA and FreeDNA for Early Non-invasive Detection and Prognosis Assessment in Urinary Bladder Cancer Patients</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neoplasma

ISSN

0028-2685

e-ISSN

—

Volume of the periodical

63

Issue of the periodical within the volume

5

Country of publishing house

SK - SLOVAKIA

Number of pages

10

Pages from-to

799-808

UT code for WoS article

000385211400018

EID of the result in the Scopus database

2-s2.0-84992343755