Morphological and gene-expression characterization of viable heterogeneous circulating tumor cells size-captured and cultured from triple-negative breast cancer mouse models

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F16%3AN0000117" target="_blank" >RIV/00064173:_____/16:N0000117 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10327685 RIV/00064203:_____/16:10327685

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Morphological and gene-expression characterization of viable heterogeneous circulating tumor cells size-captured and cultured from triple-negative breast cancer mouse models

Original language description

The goal of the present study was to capture and characterize circulating tumor cells (CTC) from mouse models of triple-negative breast cancer (TNBC), a highly recalcitrant disease. CTCs were captured by size-based filtration from orthotopic nude-mouse models of the human MDA-MB-231-GFP cell line. Subsequently, CTCs were cultured for a 3 months period in vitro. Cyto-morphological analysis with confocal microscopy demonstrated heterogeneity among the CTCs, which alternated between adherent in culture. CTC gene-expression analysis was performed with qPCR for 20 tumor-associated markers from RNA derived from adherent/nonadherent CTCs in culture. Both Fox3p and TWIST1 were highly expressed in non-adherent CTCs. The present study demonstrates the power of size-based CTC isolation from orthotopic mouse models of TNBC and GFP imaging of viable CTC cells in culture, to characterize highly-heterogeneous TNBC CTC.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

<a href="/en/project/NT14441" target="_blank" >NT14441: Circulating and disseminated tumor cells in diagnosis and treatment of advanced ovarian cancer.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Clinical and Experimental Medicine

ISSN

1940-5901

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

7772-7779

UT code for WoS article

000379956500015

EID of the result in the Scopus database

2-s2.0-84971542844