Transcript expression and genetic variability analysis of caspases in breast carcinomas suggests CASP9 as the most interesting target
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F17%3AN0000216" target="_blank" >RIV/00064173:_____/17:N0000216 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/17:43911995 RIV/00216208:11130/17:10359513 RIV/61989592:15110/17:73583822 RIV/70883521:28150/17:63518096 and 2 more
Result on the web
<a href="http://dx.doi.org/10.1515/cclm-2016-0271" target="_blank" >http://dx.doi.org/10.1515/cclm-2016-0271</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1515/cclm-2016-0271" target="_blank" >10.1515/cclm-2016-0271</a>
Alternative languages
Result language
angličtina
Original language name
Transcript expression and genetic variability analysis of caspases in breast carcinomas suggests CASP9 as the most interesting target
Original language description
BACKGROUND: Apoptosis plays a critical role in cancer cell survival and tumor development. We provide a hypothesis-generating screen for further research by exploring the expression profile and genetic variability of caspases (2, 3, 7, 8, 9, and 10) in breast carcinoma patients. This study addressed isoform-specific caspase transcript expression and genetic variability in regulatory sequences of caspases 2 and 9. METHODS: Gene expression profiling was performed by quantitative real-time PCR in tumor and paired non-malignant tissues of two independent groups of patients. Genetic variability was determined by high resolution melting, allelic discrimination, and sequencing analysis in tumor and peripheral blood lymphocyte DNA of the patients. RESULTS: CASP3 A+B and S isoforms were over-expressed in tumors of both patient groups. The CASP9 transcript was down-regulated in tumors of both groups of patients and significantly associated with expression of hormonal receptors and with the presence of rs4645978-rs2020903-rs4646034 haplotype in the CASP9 gene. Patients with a low intratumoral CASP9A/B isoform expression ratio (predicted to shift equilibrium towards anti-apoptotic isoform) subsequently treated with adjuvant chemotherapy had a significantly shorter disease-free survival than those with the high ratio (p=0.04). Inheritance of CC genotype of rs2020903 in CASP9 was associated with progesterone receptor expression in tumors (p=0.003). CONCLUSIONS: Genetic variability in CASP9 and expression of its splicing variants present targets for further study.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Chemistry and Laboratory Medicine
ISSN
1434-6621
e-ISSN
1437-4331
Volume of the periodical
55
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
111-122
UT code for WoS article
000388930700021
EID of the result in the Scopus database
2-s2.0-84998694303