The Effect of Vascular Endothelial Growth Factor C and Adipose-Derived Stem Cells on Lymphatic Regeneration in a Rat Vascularized Lymph Node Transfer Model
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F23%3A43923707" target="_blank" >RIV/00064173:_____/23:43923707 - isvavai.cz</a>
Alternative codes found
RIV/00064211:_____/23:W0000004 RIV/00216208:11110/23:10445034 RIV/00216208:11120/23:43923707 RIV/00064211:_____/23:W0000049
Result on the web
<a href="https://doi.org/10.1055/a-1896-5471" target="_blank" >https://doi.org/10.1055/a-1896-5471</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1055/a-1896-5471" target="_blank" >10.1055/a-1896-5471</a>
Alternative languages
Result language
angličtina
Original language name
The Effect of Vascular Endothelial Growth Factor C and Adipose-Derived Stem Cells on Lymphatic Regeneration in a Rat Vascularized Lymph Node Transfer Model
Original language description
Background Lymphedema is a chronic condition characterized by progressive edema with complicated treatment. Recently, new treatment strategies inducing lymphangiogenesis were proposed. The aim of our study was to examine the effect of VEGF-C and ADSC (adipose derived stem cells) on lymphatic regeneration and drainage reestablishment in vascularized lymph node transfer model using a pedicled VLN groin flap. Materials and methods Female Lewis rats with groin VLN flaps were utilized as a lymphedema model. Group A served as the control. Group B received VEGF-C. Group C received both VEGF-C and ADSCs. Group D received ADSCs only. Lymphatic drainage reestablishment was evaluated by ultrasound-photoacoustic imaging after ICG injection. Results The fastest regeneration of elevated flaps was observed in Groups B and C in all monitored periods. After the first month, ICG positivity was detected in 14.3% of animals in Group A, 71.43% of animals in Group B (OR=15; p= 0.048), and 83.33% in Group C (OR=30; p=0.027). Contrary, the difference between control group and Group D (16.67%; p=0.905) was statistically insignificant. Administration of VEGF-C, ADCs + VEGF-C, and ADSC led to full flap regeneration after six months. The control group had the lowest percentage of ICG positivity at all monitored time points. Conclusion We found that the fastest regeneration occurred with the combination of the VLN flap and VEGF-C. The addition of ADSC had an insignificant effect in our study. Furthermore, we proved the feasibility of photoacoustic imaging as an assessment tool of the lymphatic drainage recovery in a VLNT model.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30212 - Surgery
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Reconstructive Microsurgery
ISSN
0743-684X
e-ISSN
1098-8947
Volume of the periodical
39
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
311-319
UT code for WoS article
000890052300001
EID of the result in the Scopus database
2-s2.0-85134813823