SMAD2 mRNA expression is upregulated in fibrosing interstitial lung diseases (ILDs)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F15%3A%230001173" target="_blank" >RIV/00064190:_____/15:#0001173 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1183/13993003.congress-2015.PA934" target="_blank" >http://dx.doi.org/10.1183/13993003.congress-2015.PA934</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1183/13993003.congress-2015.PA934" target="_blank" >10.1183/13993003.congress-2015.PA934</a>
Alternative languages
Result language
angličtina
Original language name
SMAD2 mRNA expression is upregulated in fibrosing interstitial lung diseases (ILDs)
Original language description
Abstract ILDs are heterogenous group of diseases with different extent of inflammation and fibrosis. Fibrotic types of ILDs(IPF and NSIP) are associated with the worst prognosis. In pathogenesis of fibroproduction dysregulation of TGFbeta among others probably plays significant role. Methods: 11 patients with hypersensitivity pneumonitis(HP), 15 patients with fibrosing ILDs(IPF and NSIP) and 8 patients with connective tissue diseases (CTD) were enrolled into the prospective study. All patients underwent bronchoscopy with transbronchial biopsy during the diagnostic programme. After isolation of mRNA from lung biopsies, mRNA expression of F2RL1, GPER, IL4R, IL 13, SMAD 2, SMAD 3, TGF beta, TNF alpha, TSLP, VEGFA and YY1 were measured using TLDA cards. CT scans at the time of diagnosis were scored according to Gay S. procedure and alveolar and interstitial score was set. Groups of patients were compared using Cruscal Wallis test and relation between HRCT score and mRNA expression were estimated by Spearman correlation coefficient. Results: RNA expression of SMAD 2 was significantly higher in the group of fibrosing ILDs(median:1.90) comparing to HP(median:0.99, p=0.008) and CTD group(med.:0.98,p=0.02). Differences in other measured expressions between groups didn't reach statistical significance. Negative correlation has been found between alveolar score and SMAD 2 in all patients (p: 0.016). Conclusion: We have observed upregulated expression of SMAD 2 mRNA in the group of fibrosing ILDs. Moreover the SMAD values negatively correlated with alveolar score. We hypothesise that SMAD 2 might be used as a biomarker of fibroproliferative healing in ILDs.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FC - Pneumology
OECD FORD branch
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Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology.
ISSN
0903-1936
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
Suppl. 59
Country of publishing house
DK - DENMARK
Number of pages
3
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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